Endogenous nitric oxide is present in the exhaled air of rabbits, guinea pigs and humans

L. E. Gustafsson; A. M. Leone; M. G. Persson; N. P. Wiklund; S. Moncada

doi:10.1016/0006-291X(91)91268-H

Endogenous nitric oxide is present in the exhaled air of rabbits, guinea pigs and humans

L. E. Gustafsson, A. M. Leone, M. G. Persson, N. P. Wiklund, S. Moncada

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Abstract

The presence of nitric oxide (NO) in the exhaled air of humans and of anaesthetized rabbits and guinea pigs was demonstrated by chemiluminescence, diazotization and mass spectrometry. This NO is endogenously produced in the lung by an NO synthase, since its generation in guinea pigs and rabbits was inhibited by N^ω-nitro-L-arginine methyl ester and N^G-monomethyl-L-arginine, inhibitors of this enzyme. The effect of the inhibitors was reversed by the precursor of NO synthesis, L-arginine. Since NO is produced by normal vascular endothelium for the physiological regulation of blood flow and pressure and also by activated macrophages to contribute to non-specific immunity, our experiments suggest that NO may play both vascular regulatory and host defence roles in pulmonary physiology and pathophysiology.

Original language	English
Pages (from-to)	852-857
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	181
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(91)91268-H
State	Published - 16 Dec 1991
Externally published	Yes

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title = "Endogenous nitric oxide is present in the exhaled air of rabbits, guinea pigs and humans",

abstract = "The presence of nitric oxide (NO) in the exhaled air of humans and of anaesthetized rabbits and guinea pigs was demonstrated by chemiluminescence, diazotization and mass spectrometry. This NO is endogenously produced in the lung by an NO synthase, since its generation in guinea pigs and rabbits was inhibited by Nω-nitro-L-arginine methyl ester and NG-monomethyl-L-arginine, inhibitors of this enzyme. The effect of the inhibitors was reversed by the precursor of NO synthesis, L-arginine. Since NO is produced by normal vascular endothelium for the physiological regulation of blood flow and pressure and also by activated macrophages to contribute to non-specific immunity, our experiments suggest that NO may play both vascular regulatory and host defence roles in pulmonary physiology and pathophysiology.",

author = "Gustafsson, {L. E.} and Leone, {A. M.} and Persson, {M. G.} and Wiklund, {N. P.} and S. Moncada",

note = "Funding Information: Acknoe Supported by the Swedish Medical Research Council (proj 7919), Torsten and Ragnar Sliderberg foundations, the Swedish National Environment Protection Board (533 0051-3) and the IEM and Karolinska Institute. The authors would also like to acknowledge the assistance of Drs. M Yeadon and P. Francis.",

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doi = "10.1016/0006-291X(91)91268-H",

language = "English",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Endogenous nitric oxide is present in the exhaled air of rabbits, guinea pigs and humans

AU - Gustafsson, L. E.

AU - Leone, A. M.

AU - Persson, M. G.

AU - Wiklund, N. P.

AU - Moncada, S.

N1 - Funding Information: Acknoe Supported by the Swedish Medical Research Council (proj 7919), Torsten and Ragnar Sliderberg foundations, the Swedish National Environment Protection Board (533 0051-3) and the IEM and Karolinska Institute. The authors would also like to acknowledge the assistance of Drs. M Yeadon and P. Francis.

PY - 1991/12/16

Y1 - 1991/12/16

N2 - The presence of nitric oxide (NO) in the exhaled air of humans and of anaesthetized rabbits and guinea pigs was demonstrated by chemiluminescence, diazotization and mass spectrometry. This NO is endogenously produced in the lung by an NO synthase, since its generation in guinea pigs and rabbits was inhibited by Nω-nitro-L-arginine methyl ester and NG-monomethyl-L-arginine, inhibitors of this enzyme. The effect of the inhibitors was reversed by the precursor of NO synthesis, L-arginine. Since NO is produced by normal vascular endothelium for the physiological regulation of blood flow and pressure and also by activated macrophages to contribute to non-specific immunity, our experiments suggest that NO may play both vascular regulatory and host defence roles in pulmonary physiology and pathophysiology.

AB - The presence of nitric oxide (NO) in the exhaled air of humans and of anaesthetized rabbits and guinea pigs was demonstrated by chemiluminescence, diazotization and mass spectrometry. This NO is endogenously produced in the lung by an NO synthase, since its generation in guinea pigs and rabbits was inhibited by Nω-nitro-L-arginine methyl ester and NG-monomethyl-L-arginine, inhibitors of this enzyme. The effect of the inhibitors was reversed by the precursor of NO synthesis, L-arginine. Since NO is produced by normal vascular endothelium for the physiological regulation of blood flow and pressure and also by activated macrophages to contribute to non-specific immunity, our experiments suggest that NO may play both vascular regulatory and host defence roles in pulmonary physiology and pathophysiology.

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U2 - 10.1016/0006-291X(91)91268-H

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EP - 857

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Endogenous nitric oxide is present in the exhaled air of rabbits, guinea pigs and humans

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