@article{9037449885174661b5f84d7e79633dcb,
title = "Endocytosis of the TCR/CD3 complex and the class-I major histocompatibility complex in a human T cell line",
abstract = "We investigated the expression of the T cell receptor (TCR)/CD3 complex on a CD4-positive human T cell lymphoma cell line treated with phorbol myristate acetate (PMA) and/or Ca2+ ionophore using fluorescence flow cytometry and fluorescence microscopic analysis. PMA induced a significant decrease in the expression of the CD3 complex on the cell membranes. Fluorescence microscopy confirmed that the down regulation is due to internalization of the antigens. Ca2+ ionophore treatment had no effect on the internalization of the CD3 complex. Double staining revealed that the vesicles containing the internalized CD3 complex and those containing intracytoplasmic class I major histocompatibility complex antigen had similar distribution in the PMA-stimulated cells, implying coexistence of these two antigens in a cytoplasmic perinuclear distribution.",
author = "Yasuhiro Yamane and Maritza Perez and Richard Edelson and Nicodemo Agostino and Benvenuto Pernis",
note = "Funding Information: Endocytosis of the class I major histocompatibility complex (MHC-I) antigens and the T cell receptor (TCR)/CD3 complex on T cells has been the subject of recent {\textquoteright} This work was supported in part by Nattonal lnstltutes of Health Grant CA-43058-06 and by Muittple Sclerosis Sot. PP 0 112. {\textquoteright} To whom correspondence and reprint requests should be addressed. {\textquoteright} Abbreviations used: TCR, T cell receptor; MHC-I, class I major histocompatibitity complex; PMA, phorbol my&ate acetate: MoAb, monoclonal antibody: FITC, fluorescein isothiocyanate: TRITC. tetramethyl rhodamine isothiocyanate; Con A, concanavalin .A: MFI, mean fluorescence intensity: PBS, phosphate-buffered saline.",
year = "1991",
month = sep,
doi = "10.1016/0008-8749(91)90370-Q",
language = "English",
volume = "136",
pages = "496--503",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "2",
}