Employing nanobodies for immune landscape profiling by PET imaging in mice

Abraham J.P. Teunissen, Omar B. Abousaway, Jazz Munitz, Mandy M.T. van Leent, Yohana C. Toner, Bram Priem, Max L. Senders, Carlos Pérez-Medina, Willem J.M. Mulder, Mohammad Rashidian

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Noninvasive immunoimaging holds great potential for studying and stratifying disease as well as therapeutic efficacy. Radiolabeled single-domain antibody fragments (i.e., nanobodies) are appealing probes for immune landscape profiling, as they display high stability, rapid targeting, and excellent specificity, while allowing extremely sensitive nuclear readouts. Here, we present a protocol for radiolabeling an anti-CD11b nanobody and studying its uptake in mice by a combination of positron emission tomography imaging, ex vivo gamma counting, and autoradiography. Our protocol is applicable to nanobodies against other antigens. For complete details on the use and execution of this protocol, please see Priem et al. (2020), Senders et al. (2019), or Rashidian et al. (2017).

Original languageEnglish
Article number100434
JournalSTAR Protocols
Issue number2
StatePublished - 18 Jun 2021


  • Antibody
  • Immunology
  • Model Organisms
  • Molecular Biology
  • Molecular/Chemical Probes


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