Elongated mouse chromosomes suitable for enhanced molecular cytogenetics

Sherif Louis; Katalin Benedek; Michael Mowat; George Klein; Sabine Mai

doi:10.1007/s10616-004-2978-2

Elongated mouse chromosomes suitable for enhanced molecular cytogenetics

Sherif Louis, Katalin Benedek, Michael Mowat, George Klein, Sabine Mai

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Characterization of genetic disorders in humans and animal models requires identification of chromosomal aberrations. However, identifying fine deletions or insertion in metaphase chromosomes has been always a challenge due to limitations of resolution. In this study we developed a rapid method for chromosome elongation using two different intercalating agents: ethidium bromide and 5-bromo-2′-deoxyuridine (BrdU), together with a short-term mitotic block using colcemid. About 70% of the chromosomes from cells that underwent this elongation procedure reached three times longer than those prepared from control cells. FISH experiments using elongated chromosomes revealed a duplicated region of chromosome 11 that was not visible in cells prepared with conventional methods.

Original language	English
Pages (from-to)	143-149
Number of pages	7
Journal	Cytotechnology
Volume	44
Issue number	3
DOIs	https://doi.org/10.1007/s10616-004-2978-2
State	Published - Dec 2004
Externally published	Yes

Keywords

Elongated chromosomes
Intercalating agents
Mitotic block
Molecular cytogenetics

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10.1007/s10616-004-2978-2

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title = "Elongated mouse chromosomes suitable for enhanced molecular cytogenetics",

abstract = "Characterization of genetic disorders in humans and animal models requires identification of chromosomal aberrations. However, identifying fine deletions or insertion in metaphase chromosomes has been always a challenge due to limitations of resolution. In this study we developed a rapid method for chromosome elongation using two different intercalating agents: ethidium bromide and 5-bromo-2′-deoxyuridine (BrdU), together with a short-term mitotic block using colcemid. About 70% of the chromosomes from cells that underwent this elongation procedure reached three times longer than those prepared from control cells. FISH experiments using elongated chromosomes revealed a duplicated region of chromosome 11 that was not visible in cells prepared with conventional methods.",

keywords = "Elongated chromosomes, Intercalating agents, Mitotic block, Molecular cytogenetics",

author = "Sherif Louis and Katalin Benedek and Michael Mowat and George Klein and Sabine Mai",

note = "Funding Information: The authors thank Dr Francis Wiener for discussions and critical reading of this manuscript. This study was performed with financial support from NSERC (Canada) and Cancer Research Institute/ Concern Foundation (Sweden). SL is funded through a fellowship funded by the CIHR Strategic Training Program entitled {\textquoteleft}{\textquoteleft}Innovative Technologies in Multidisciplinary Health Research Trainng{\textquoteright}{\textquoteright}.",

year = "2004",

month = dec,

doi = "10.1007/s10616-004-2978-2",

language = "English",

volume = "44",

pages = "143--149",

journal = "Cytotechnology",

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T1 - Elongated mouse chromosomes suitable for enhanced molecular cytogenetics

AU - Louis, Sherif

AU - Benedek, Katalin

AU - Mowat, Michael

AU - Klein, George

AU - Mai, Sabine

N1 - Funding Information: The authors thank Dr Francis Wiener for discussions and critical reading of this manuscript. This study was performed with financial support from NSERC (Canada) and Cancer Research Institute/ Concern Foundation (Sweden). SL is funded through a fellowship funded by the CIHR Strategic Training Program entitled ‘‘Innovative Technologies in Multidisciplinary Health Research Trainng’’.

PY - 2004/12

Y1 - 2004/12

N2 - Characterization of genetic disorders in humans and animal models requires identification of chromosomal aberrations. However, identifying fine deletions or insertion in metaphase chromosomes has been always a challenge due to limitations of resolution. In this study we developed a rapid method for chromosome elongation using two different intercalating agents: ethidium bromide and 5-bromo-2′-deoxyuridine (BrdU), together with a short-term mitotic block using colcemid. About 70% of the chromosomes from cells that underwent this elongation procedure reached three times longer than those prepared from control cells. FISH experiments using elongated chromosomes revealed a duplicated region of chromosome 11 that was not visible in cells prepared with conventional methods.

AB - Characterization of genetic disorders in humans and animal models requires identification of chromosomal aberrations. However, identifying fine deletions or insertion in metaphase chromosomes has been always a challenge due to limitations of resolution. In this study we developed a rapid method for chromosome elongation using two different intercalating agents: ethidium bromide and 5-bromo-2′-deoxyuridine (BrdU), together with a short-term mitotic block using colcemid. About 70% of the chromosomes from cells that underwent this elongation procedure reached three times longer than those prepared from control cells. FISH experiments using elongated chromosomes revealed a duplicated region of chromosome 11 that was not visible in cells prepared with conventional methods.

KW - Elongated chromosomes

KW - Intercalating agents

KW - Mitotic block

KW - Molecular cytogenetics

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U2 - 10.1007/s10616-004-2978-2

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JO - Cytotechnology

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ER -

Elongated mouse chromosomes suitable for enhanced molecular cytogenetics

Abstract

Keywords

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