Elemental bio-imaging of melanoma in lymph node biopsies

Dominic Hare; Fiona Burger; Christine Austin; Fred Fryer; Rudolf Grimm; Brian Reedy; Richard A. Scolyer; John F. Thompson; Philip Doble

doi:10.1039/b812745j

Elemental bio-imaging of melanoma in lymph node biopsies

Dominic Hare, Fiona Burger, Christine Austin, Fred Fryer, Rudolf Grimm, Brian Reedy, Richard A. Scolyer, John F. Thompson, Philip Doble

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

The spatial distribution of trace elements in human lymph nodes partially infiltrated by melanoma cells was determined by elemental bio-imaging. Imaging of ³¹P within the nodal capsule and normal lymph node tissue showed a clear demarcation of the tumour boundary, with a significant decrease in relative ³¹P concentration within the tumour. The location of the tumour boundary was confirmed by haematoxylin and eosin staining of serial sections and observation by light microscopy. Further enhancement of the tumour boundary was achieved by imaging the ³¹P/³⁴S ratio. ³¹P/⁶⁶Zn ratio images showed a decreasing ratio beyond the tumour boundary that extended into peritumour normal lymph node tissue.

Original language	English
Pages (from-to)	450-453
Number of pages	4
Journal	The Analyst
Volume	134
Issue number	3
DOIs	https://doi.org/10.1039/b812745j
State	Published - 2009
Externally published	Yes

Access to Document

10.1039/b812745j

Cite this

@article{e247c0d79f3040b189f70ae12352f7b8,

title = "Elemental bio-imaging of melanoma in lymph node biopsies",

abstract = "The spatial distribution of trace elements in human lymph nodes partially infiltrated by melanoma cells was determined by elemental bio-imaging. Imaging of 31P within the nodal capsule and normal lymph node tissue showed a clear demarcation of the tumour boundary, with a significant decrease in relative 31P concentration within the tumour. The location of the tumour boundary was confirmed by haematoxylin and eosin staining of serial sections and observation by light microscopy. Further enhancement of the tumour boundary was achieved by imaging the 31P/34S ratio. 31P/66Zn ratio images showed a decreasing ratio beyond the tumour boundary that extended into peritumour normal lymph node tissue.",

author = "Dominic Hare and Fiona Burger and Christine Austin and Fred Fryer and Rudolf Grimm and Brian Reedy and Scolyer, {Richard A.} and Thompson, {John F.} and Philip Doble",

year = "2009",

doi = "10.1039/b812745j",

language = "English",

volume = "134",

pages = "450--453",

journal = "The Analyst",

issn = "0003-2654",

publisher = "Royal Society of Chemistry",

number = "3",

}

TY - JOUR

T1 - Elemental bio-imaging of melanoma in lymph node biopsies

AU - Hare, Dominic

AU - Burger, Fiona

AU - Austin, Christine

AU - Fryer, Fred

AU - Grimm, Rudolf

AU - Reedy, Brian

AU - Scolyer, Richard A.

AU - Thompson, John F.

AU - Doble, Philip

PY - 2009

Y1 - 2009

N2 - The spatial distribution of trace elements in human lymph nodes partially infiltrated by melanoma cells was determined by elemental bio-imaging. Imaging of 31P within the nodal capsule and normal lymph node tissue showed a clear demarcation of the tumour boundary, with a significant decrease in relative 31P concentration within the tumour. The location of the tumour boundary was confirmed by haematoxylin and eosin staining of serial sections and observation by light microscopy. Further enhancement of the tumour boundary was achieved by imaging the 31P/34S ratio. 31P/66Zn ratio images showed a decreasing ratio beyond the tumour boundary that extended into peritumour normal lymph node tissue.

AB - The spatial distribution of trace elements in human lymph nodes partially infiltrated by melanoma cells was determined by elemental bio-imaging. Imaging of 31P within the nodal capsule and normal lymph node tissue showed a clear demarcation of the tumour boundary, with a significant decrease in relative 31P concentration within the tumour. The location of the tumour boundary was confirmed by haematoxylin and eosin staining of serial sections and observation by light microscopy. Further enhancement of the tumour boundary was achieved by imaging the 31P/34S ratio. 31P/66Zn ratio images showed a decreasing ratio beyond the tumour boundary that extended into peritumour normal lymph node tissue.

UR - http://www.scopus.com/inward/record.url?scp=60649112013&partnerID=8YFLogxK

U2 - 10.1039/b812745j

DO - 10.1039/b812745j

M3 - Article

C2 - 19238278

AN - SCOPUS:60649112013

SN - 0003-2654

VL - 134

SP - 450

EP - 453

JO - The Analyst

JF - The Analyst

IS - 3

ER -

Elemental bio-imaging of melanoma in lymph node biopsies

Abstract

Access to Document

Fingerprint

Cite this