Elemental bio-imaging of melanoma in lymph node biopsies

Dominic Hare, Fiona Burger, Christine Austin, Fred Fryer, Rudolf Grimm, Brian Reedy, Richard A. Scolyer, John F. Thompson, Philip Doble

Research output: Contribution to journalArticlepeer-review

47 Scopus citations


The spatial distribution of trace elements in human lymph nodes partially infiltrated by melanoma cells was determined by elemental bio-imaging. Imaging of 31P within the nodal capsule and normal lymph node tissue showed a clear demarcation of the tumour boundary, with a significant decrease in relative 31P concentration within the tumour. The location of the tumour boundary was confirmed by haematoxylin and eosin staining of serial sections and observation by light microscopy. Further enhancement of the tumour boundary was achieved by imaging the 31P/34S ratio. 31P/66Zn ratio images showed a decreasing ratio beyond the tumour boundary that extended into peritumour normal lymph node tissue.

Original languageEnglish
Pages (from-to)450-453
Number of pages4
JournalThe Analyst
Issue number3
StatePublished - 2009
Externally publishedYes


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