Element analysis in hair by x-ray fluorescence

L. Zeitz, R. Lee, E. O. Rothschild

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


An x-ray fluorescence method is described that allows for the quantitative determination of the weight fraction of zinc and calcium and the semiquantitative determination of sulfur in a single strand of human hair. An analytical technique that includes absolute calibration is presented and the self-absorption effect analyzed. The limit of detectability of the method is about 0.024 mg zinc per gram of hair for zinc and 0.020 mg calcium per gram of hair for calcium in a 10 minute integration. Modifications are suggested which should lower these values by at least a factor of 10. A washing procedure to be used in preparing hair for element analysis in projected biomedical research studies is described.

Original languageEnglish
Pages (from-to)123-132
Number of pages10
JournalAnalytical Biochemistry
Issue numberC
StatePublished - 1969
Externally publishedYes


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