Electron microscopic demonstration of two types of mitochondria with different affinities for lead

Patricia D. Wilson

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Incubation of glutaraldehyde-fixed, non-frozen tissue slices of mouse seminal vesicle, ventral prostate and small intestine in the media described by Hugon & Borgers (1966) and by Mayahara et al. (1967) for the cytochemical demonstration of alkaline phosphatase resulted in the deposition of lead along smooth muscle mitochondrial membranes but not along epithelial mitochondrial membranes. Control studies using boiled tissues; media lacking substrate; inhibitors such as L-cysteine, EDTA, N-ethyl maleimide and p-chloromercuribenzoate; and tissues incubated in full media after fixation, embedding and sectioning, showed that the reaction in muscle mitochondria was non-enzymatic. It was concluded that the phospholipid component of muscle mitochondrial membranes differed from that of epithelial mitochondrial membranes.

Original languageEnglish
Pages (from-to)405-416
Number of pages12
JournalJournal of Molecular Histology
Volume1
Issue number5
DOIs
StatePublished - Aug 1969
Externally publishedYes

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