Electron capture dissociation for robust production of sequence tags from eukaryotic proteins

Neil L. Kelleher, Steven Patrie, Jeffrey R. Johnson, Fanyu Meng, Andrew J. Forbes

Research output: Contribution to conferencePaperpeer-review

Abstract

The potential of electron capture dissociation (ECD) was translated into identification of heavily-modified proteins from eukaryotic proteins isolated from S. cerevisiae. Yeast cells were grown to stationary phase and collected by centrifugation. Cell lysis by sonication was followed by fractionation of the cell extract using a combination of continuous-elution PAGE and reversed-phase HPLC. It was found that ∼40-50% of fragment ions from standard threshold dissociation techniques do not occur in strings, for ions of ∼75 wild-type proteins.

Original languageEnglish
Pages101-102
Number of pages2
StatePublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2 Jun 20026 Jun 2002

Conference

ConferenceProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/TerritoryUnited States
CityOrlando, FL
Period2/06/026/06/02

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