Electrically silent Na+ and Cl- fluxes across the rabbit ciliary epithelium

T. C. Chu, O. A. Candia

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Abstract

Unidirectional Na+, Cl-, and mannitol fluxes were measured across the isolated and short-circuited rabbit iris-ciliary body as functions of the Cl- concentration in the bathing solutions (0, 26 and 80 mM). At constant Na+ concentration, Na+ fluxes increased on the average from 8.0 to 11.9 μeq/hr as the Cl- concentration was raised and vice versa. Cl- fluxes also increased more than expected from simple diffusion; from 2.0 (26 mM) to 8.1 μeq/hr (80 mM). Mannitol permeability (9.0 x 10-7 cm/sec) was independent of the Cl- concentration and similar to that measured in 'tight' epithelia. In Cl--free solutions, there was good agreement between the measured electrical resistance and that calculared with the partial conductance equation. In Cl--rich solutions, the calculated resistance was smaller than the measured resistance, suggesting electrically silent fluxes of Na+ and Cl-. These silent fluxes were of similar magnitude and possibly coupled with each other. This mechanism may provide an additional pathway for electrolyte movement across the ciliary epithelium.

Original languageEnglish
Pages (from-to)445-450
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume28
Issue number3
StatePublished - 1987

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