TY - JOUR
T1 - Electrical aggregometry in whole blood from human, pig and rabbit
AU - Galvez, A.
AU - Badimon, L.
AU - Badimon, J. J.
AU - Fuster, V.
PY - 1986
Y1 - 1986
N2 - The objective of this study was to characterize and standardize whole blood electrical aggregometry (WBEA) in the pig and rabbit, animal models extensively used in atherosclerosis research, and to compare their platelet response with that of man. Platelet aggregation was studied in blood (WBEA) and platelet rich plasma (optical aggregometry, OA). Dose response curves were obtained for ADP and collagen. The effect of hematocrit on WBEA was also evaluated. Aggregation with ADP and collagen using WBEA was more extensive with human than with pig or rabbit platelets. OA revealed similar differences among species but the time to reach maximal aggregation was markedly shorter. Using WBEA, the extent of aggregation was inversely related to the hematocrit. We conclude that WBEA is a useful technique that may be of particular importance in situations where hyperlipidemic plasma prevents the use of OA, as occurs in some atherosclerosis research animal models.
AB - The objective of this study was to characterize and standardize whole blood electrical aggregometry (WBEA) in the pig and rabbit, animal models extensively used in atherosclerosis research, and to compare their platelet response with that of man. Platelet aggregation was studied in blood (WBEA) and platelet rich plasma (optical aggregometry, OA). Dose response curves were obtained for ADP and collagen. The effect of hematocrit on WBEA was also evaluated. Aggregation with ADP and collagen using WBEA was more extensive with human than with pig or rabbit platelets. OA revealed similar differences among species but the time to reach maximal aggregation was markedly shorter. Using WBEA, the extent of aggregation was inversely related to the hematocrit. We conclude that WBEA is a useful technique that may be of particular importance in situations where hyperlipidemic plasma prevents the use of OA, as occurs in some atherosclerosis research animal models.
UR - http://www.scopus.com/inward/record.url?scp=0023009887&partnerID=8YFLogxK
U2 - 10.1055/s-0038-1661625
DO - 10.1055/s-0038-1661625
M3 - Article
C2 - 3810551
AN - SCOPUS:0023009887
SN - 0340-6245
VL - 56
SP - 128
EP - 132
JO - Thrombosis and Haemostasis
JF - Thrombosis and Haemostasis
IS - 2
ER -