TY - JOUR
T1 - Egg-sperm interactions at fertilization in mammals
AU - Wassarman, Paul M.
AU - Jovine, Luca
AU - Litscher, Eveline S.
AU - Qi, Huayu
AU - Williams, Zev
N1 - Funding Information:
We are grateful to Serono Symposia International for enabling us to participate in this symposium. We thank all of those who came before us in our laboratory for providing a firm foundation on which to build. L. Jovine is supported by a Human Frontier Science Program long-term fellowship. Some of the research described here was supported in part by the NIH (HD-35105).
PY - 2004/7/1
Y1 - 2004/7/1
N2 - Mammalian eggs are surrounded by a zona pellucida (ZP) that regulates egg-sperm teractions during fertilization. The ZP consists of long filaments composed of two glycoproteins, ZP2 and ZP3, that are crosslinked by a third glycoprotein, ZP1. The presence of both ZP2 and ZP3 is essential for assembling a ZP around growing oocytes, as well as for fertility of females. Acrosome-intact sperm recognize and bind to O-linked oligosaccharides linked to Ser residues at the sperm combining-site of ZP3. Structural differences in oligosaccharides on ZP3 from different species may account for whether or not sperm are able to bind to the ZP. Bound sperm undergo the acrosome reaction, penetrate the ZP, and can then fuse with egg plasma membrane. Following fertilization, sperm are unable to bind to either ZP3 or the ZP of one-cell embryos.
AB - Mammalian eggs are surrounded by a zona pellucida (ZP) that regulates egg-sperm teractions during fertilization. The ZP consists of long filaments composed of two glycoproteins, ZP2 and ZP3, that are crosslinked by a third glycoprotein, ZP1. The presence of both ZP2 and ZP3 is essential for assembling a ZP around growing oocytes, as well as for fertility of females. Acrosome-intact sperm recognize and bind to O-linked oligosaccharides linked to Ser residues at the sperm combining-site of ZP3. Structural differences in oligosaccharides on ZP3 from different species may account for whether or not sperm are able to bind to the ZP. Bound sperm undergo the acrosome reaction, penetrate the ZP, and can then fuse with egg plasma membrane. Following fertilization, sperm are unable to bind to either ZP3 or the ZP of one-cell embryos.
KW - Acrosome reaction
KW - Eggs
KW - Fertilization
KW - Sperm
KW - ZP glycoproteins
KW - Zona pellucida
UR - http://www.scopus.com/inward/record.url?scp=2942511366&partnerID=8YFLogxK
U2 - 10.1016/j.ejogrb.2004.01.025
DO - 10.1016/j.ejogrb.2004.01.025
M3 - Conference article
C2 - 15196717
AN - SCOPUS:2942511366
SN - 0301-2115
VL - 115
SP - S57-S60
JO - European Journal of Obstetrics, Gynecology and Reproductive Biology
JF - European Journal of Obstetrics, Gynecology and Reproductive Biology
IS - SUPPL.
T2 - Genesis and Fate of the Preimplantation Embryo
Y2 - 29 September 2003 through 1 October 2003
ER -