Efficient retroviral transfer of a mouse c-myc construct into HL60

Gábor Szabó; Gösta Winberg; Marie Henriksson; George Klein; János Sümegi

doi:10.1016/0006-291X(89)92138-4

Efficient retroviral transfer of a mouse c-myc construct into HL60

Gábor Szabó, Gösta Winberg, Marie Henriksson, George Klein, János Sümegi

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Abstract

We introduced an LTR-driven mouse c-myc second and third exon, Tn5Neo gene construct into the inducible human leukemia line HL60 using an amphotropic retroviral vector system. Over 90 % of the cells became neo-resistant and the transfected myc gene was transcribed in several neomycin resistant clones. Making use of the simultaneous presence of the different myc genes in the same cell, we compared expression of the corresponding mRNAs after differentiation and their decay mechanisms.

Original language	English
Pages (from-to)	321-327
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	163
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(89)92138-4
State	Published - 30 Aug 1989
Externally published	Yes

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abstract = "We introduced an LTR-driven mouse c-myc second and third exon, Tn5Neo gene construct into the inducible human leukemia line HL60 using an amphotropic retroviral vector system. Over 90 % of the cells became neo-resistant and the transfected myc gene was transcribed in several neomycin resistant clones. Making use of the simultaneous presence of the different myc genes in the same cell, we compared expression of the corresponding mRNAs after differentiation and their decay mechanisms.",

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AU - Henriksson, Marie

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AU - Sümegi, János

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AB - We introduced an LTR-driven mouse c-myc second and third exon, Tn5Neo gene construct into the inducible human leukemia line HL60 using an amphotropic retroviral vector system. Over 90 % of the cells became neo-resistant and the transfected myc gene was transcribed in several neomycin resistant clones. Making use of the simultaneous presence of the different myc genes in the same cell, we compared expression of the corresponding mRNAs after differentiation and their decay mechanisms.

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Efficient retroviral transfer of a mouse c-myc construct into HL60

Abstract

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