Efficient in vitro expansion of JC virus-specific CD8+ T-cell responses by JCV peptide-stimulated dendritic cells from patients with progressive multifocal leukoencephalopathy

Angela Marzocchetti, Marco Lima, Troy Tompkins, Daniel G. Kavanagh, Rajesh T. Gandhi, David W. O'Neill, Nina Bhardwaj, Igor J. Koralnik

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the brain caused by JC virus (JCV) for which there is no cure. PML patients who have JCV-specific CD8+ cytotoxic T lymphocytes (CTL) in their blood have a better clinical outcome. We compared JCV-specific CTL responses in vitro elicited either by JCV peptide-loaded dendritic cells (DC) or by direct peptide stimulation of lymphocytes from 20 HLA-A*0201+ healthy controls, HIV+ and PML patients. JCV peptide-loaded DC elicited a stronger CTL expansion in 13/15 responders. DC can induce a potent JCV-specific CTL response in vitro, and may constitute a promising approach for PML immunotherapy.

Original languageEnglish
Pages (from-to)173-177
Number of pages5
JournalVirology
Volume383
Issue number2
DOIs
StatePublished - 20 Jan 2009
Externally publishedYes

Keywords

  • Dendritic cells
  • JC virus
  • Progressive multifocal leukoencephalopathy

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