@inbook{26985769a9134bd8998d3f5730a87b3c,
title = "Efficient gene knockdowns in mouse embryonic stem cells using microRNA-based shRNAs",
abstract = "RNA interference (RNAi) is a powerful gene-knockdown technology that has been applied for functional genetic loss-of-function studies in many model eukaryotic systems, including embryonic stem cells (ESCs). Application of RNAi in ESCs allows for dissection of mechanisms by which ESCs self-renew and maintain pluripotency, and also specifying particular cell types needed for cell-replacement therapies. Potent RNAi response can be induced by expression of an microRNA-embedded short-hairpin RNA (shRNA mir) cassette that is integrated in the genome by virus infection or site-specific recombination at a defined locus. In this chapter, I will provide detailed protocols to perform shRNA mir-mediated RNAi studies in mouse ESCs using retrovirus infection and loxP site-directed recombination for efficient constitutive and inducible gene knockdown, respectively.",
keywords = "RNA interference, embryonic stem cells, microRNA, microRNA-embedded short-hairpin RNA (shRNA )",
author = "Jianlong Wang",
year = "2010",
doi = "10.1007/978-1-60761-769-3_18",
language = "English",
isbn = "9781607617686",
series = "Methods in Molecular Biology",
pages = "241--256",
editor = "Baohong Zhang and Edmund Stellwag",
booktitle = "RNAi and MicroRNA-Mediated Gene Regulation in Stem Cells",
}