@inbook{be82284a2bac49dd808c36e014277340,
title = "Efficient gene knockdowns in mouse embryonic stem cells using MicroRNA-based shRNAs",
abstract = "RNA interference (RNAi) is a powerful gene knockdown technology that has been applied for functional genetic loss-of-function studies in many model eukaryotic systems, including embryonic stem cells (ESCs). Application of RNAi in ESCs allows for dissection of mechanisms by which ESCs self-renew and maintain pluripotency and also for specifying particular cell types needed for cell replacement therapies. Potent RNAi response can be induced by expression of a microRNA-embedded short-hairpin RNA (shRNAmir) cassette that is integrated in the genome by virus infection or site-specific recombination at a defined locus. In this chapter, I will provide detailed protocols to perform shRNAmir-mediated RNAi studies in mouse ESCs using retrovirus infection and loxP site-directed recombination for efficient constitutive and inducible gene knockdown, respectively.",
keywords = "Embryonic stem cells, MicroRNA, MicroRNA-embedded short-hairpin RNA (shRNA), RNA interference",
author = "Jianlong Wang",
note = "Publisher Copyright: {\textcopyright} 2017, Springer Science+Business Media LLC.",
year = "2017",
doi = "10.1007/978-1-4939-7108-4_17",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "241--254",
booktitle = "Methods in Molecular Biology",
}