TY - JOUR
T1 - Efficacy of expired foot-and-mouth disease O type vaccines in cattle and buffalo in Lao People's Democratic Republic
AU - Sakamoto, Kenichi
AU - Morioka, Kazuki
AU - Fukai, Katsuhiko
AU - Yamamoto, Takehisa
AU - Tsutsui, Toshiyuki
AU - Muroga, Norihiko
AU - Ojima, Noriyoshi
AU - Mago, Josuke
AU - Katagiri, Yoshito
AU - Aviso, Sharie R.
AU - Phouaravanh, Manivanh
AU - Soukvilay, Vilayvanh
AU - Keokhamphet, Chattouphone
AU - Buranathai, Chantanee
AU - Khounsy, Syseng
AU - Yamada, Manabu
PY - 2016/4/1
Y1 - 2016/4/1
N2 - Lao People's Democratic Republic (Lao PDR) submitted a request to Japan for 200,000 doses of expired foot-and-mouth disease (FMD) O type vaccines that were in storage for emergency use. Approximately 100,000 animals, consisting of both cattle and Asian water buffalo (Bubalus bubalus bubalis), received the same vaccine twice within one month in Xieng Khouang province in the northeast area of Lao PDR. Concurrently, the efficacy of three-month expired FMD O type vaccine (6PD50 O Manisa) was assessed in serum samples of 90 cattle and 31 buffalo from the field using a Liquid Phase Blocking-ELISA (LPBE) assay. Of these samples, 75 cattle (83.3%) and 24 buffalo (77.4%) were seropositive against the FMD virus (FMDV) O type before vaccination. Testing for non-structural protein (NSP) using the PrioCHECK FMD NS kit showed that many of the animals with high titers in the screening test before vaccination were FMDV-infected animals. Fifteen cattle and seven buffalo with titers 1:32 or under before vaccination exhibited high titers of antibody (1:45-1:1448) one month after the first vaccination and further increased titers (1:362-1:5792) one month after the second vaccination. Nearly all of the cattle (97.6%) had high titers to control FMD 14 months after the second vaccination. To date, no outbreak of FMD has been reported at the study site. Three-month expired FMD O type vaccines induced appropriate immune responses against FMD in both cattle and buffalo.
AB - Lao People's Democratic Republic (Lao PDR) submitted a request to Japan for 200,000 doses of expired foot-and-mouth disease (FMD) O type vaccines that were in storage for emergency use. Approximately 100,000 animals, consisting of both cattle and Asian water buffalo (Bubalus bubalus bubalis), received the same vaccine twice within one month in Xieng Khouang province in the northeast area of Lao PDR. Concurrently, the efficacy of three-month expired FMD O type vaccine (6PD50 O Manisa) was assessed in serum samples of 90 cattle and 31 buffalo from the field using a Liquid Phase Blocking-ELISA (LPBE) assay. Of these samples, 75 cattle (83.3%) and 24 buffalo (77.4%) were seropositive against the FMD virus (FMDV) O type before vaccination. Testing for non-structural protein (NSP) using the PrioCHECK FMD NS kit showed that many of the animals with high titers in the screening test before vaccination were FMDV-infected animals. Fifteen cattle and seven buffalo with titers 1:32 or under before vaccination exhibited high titers of antibody (1:45-1:1448) one month after the first vaccination and further increased titers (1:362-1:5792) one month after the second vaccination. Nearly all of the cattle (97.6%) had high titers to control FMD 14 months after the second vaccination. To date, no outbreak of FMD has been reported at the study site. Three-month expired FMD O type vaccines induced appropriate immune responses against FMD in both cattle and buffalo.
KW - Foot-and-mouth disease virus
KW - Liquid-phase blocking ELISA
KW - Serology
UR - http://www.scopus.com/inward/record.url?scp=84966709102&partnerID=8YFLogxK
U2 - 10.6090/jarq.50.163
DO - 10.6090/jarq.50.163
M3 - Article
AN - SCOPUS:84966709102
SN - 0021-3551
VL - 50
SP - 163
EP - 168
JO - Japan Agricultural Research Quarterly
JF - Japan Agricultural Research Quarterly
IS - 2
ER -