Efficacy of a 365 nm Ultraviolet A1 light Emitting Diode (UVA1-LED) in in vitro Extracorporeal Photopheresis

Akira Maeda; Riho Yamamoto; Shohei Mizuno; Shigeichiro Miki; Yasuhiro Sakamoto; Shuhei Kogata; Chiyoshi Toyama; Kazuki Sato; Chizu Okamatsu; Takanori Ando; Minako Iida; Toru Watsuji; Toshinobu Sato; Shuji Miyagawa; Hiroomi Okuyama; Akiyoshi Takami; Yoshitaka Kodera

doi:10.1111/php.13613

Efficacy of a 365 nm Ultraviolet A1 light Emitting Diode (UVA1-LED) in in vitro Extracorporeal Photopheresis

Akira Maeda, Riho Yamamoto, Shohei Mizuno, Shigeichiro Miki, Yasuhiro Sakamoto, Shuhei Kogata, Chiyoshi Toyama, Kazuki Sato, Chizu Okamatsu, Takanori Ando, Minako Iida, Toru Watsuji, Toshinobu Sato, Shuji Miyagawa, Hiroomi Okuyama, Akiyoshi Takami, Yoshitaka Kodera

Research output: Contribution to journal › Article › peer-review

Abstract

Extracorporeal photochemotherapy (ECP) is one of the more effective cell therapies for graft-versus-host disease (GvHD). ECP is a widely recommended therapeutic approach for the treatment of chronic GvHD, particularly steroid-refractory GVHD. In recent years, the use of a light emitting diode (LED) in the clinic has attracted considerable interest. In this study, we examined the issue of whether an ultraviolet A1-light emitting diode (UVA1-LED) can be used as a light source in ECP. To compare the efficacy of ECP with conventional UVA lamp and a UVA1-LED, we established an in vitro ECP model. Treatment efficacy was evaluated by measuring the % apoptosis and the inhibition of T-cell proliferation. To investigate the effect of ECP on the innate immune reaction, THP-1 cells with a luciferase reporter gene driven by a NF-kB response element (THP-1 luc NF-kB) were treated with ECP. The LED-ECP induced apoptosis and inhibition of T-cell proliferation as efficiently as a conventional ECP. However, LED-ECP induced less innate immunity in THP-1. Since LED devices are more compact compared with conventional UVA irradiation devices, the use of a UVA1-LED in the treatment of ECP may be a better alternative to conventional ECP therapy.

Original language	English
Pages (from-to)	1229-1235
Number of pages	7
Journal	Photochemistry and Photobiology
Volume	98
Issue number	5
DOIs	https://doi.org/10.1111/php.13613
State	Published - 1 Sep 2022
Externally published	Yes

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Maeda, A., Yamamoto, R., Mizuno, S., Miki, S., Sakamoto, Y., Kogata, S., Toyama, C., Sato, K., Okamatsu, C., Ando, T., Iida, M., Watsuji, T., Sato, T., Miyagawa, S., Okuyama, H., Takami, A., & Kodera, Y. (2022). Efficacy of a 365 nm Ultraviolet A1 light Emitting Diode (UVA1-LED) in in vitro Extracorporeal Photopheresis. Photochemistry and Photobiology, 98(5), 1229-1235. https://doi.org/10.1111/php.13613

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title = "Efficacy of a 365 nm Ultraviolet A1 light Emitting Diode (UVA1-LED) in in vitro Extracorporeal Photopheresis",

abstract = "Extracorporeal photochemotherapy (ECP) is one of the more effective cell therapies for graft-versus-host disease (GvHD). ECP is a widely recommended therapeutic approach for the treatment of chronic GvHD, particularly steroid-refractory GVHD. In recent years, the use of a light emitting diode (LED) in the clinic has attracted considerable interest. In this study, we examined the issue of whether an ultraviolet A1-light emitting diode (UVA1-LED) can be used as a light source in ECP. To compare the efficacy of ECP with conventional UVA lamp and a UVA1-LED, we established an in vitro ECP model. Treatment efficacy was evaluated by measuring the % apoptosis and the inhibition of T-cell proliferation. To investigate the effect of ECP on the innate immune reaction, THP-1 cells with a luciferase reporter gene driven by a NF-kB response element (THP-1 luc NF-kB) were treated with ECP. The LED-ECP induced apoptosis and inhibition of T-cell proliferation as efficiently as a conventional ECP. However, LED-ECP induced less innate immunity in THP-1. Since LED devices are more compact compared with conventional UVA irradiation devices, the use of a UVA1-LED in the treatment of ECP may be a better alternative to conventional ECP therapy.",

author = "Akira Maeda and Riho Yamamoto and Shohei Mizuno and Shigeichiro Miki and Yasuhiro Sakamoto and Shuhei Kogata and Chiyoshi Toyama and Kazuki Sato and Chizu Okamatsu and Takanori Ando and Minako Iida and Toru Watsuji and Toshinobu Sato and Shuji Miyagawa and Hiroomi Okuyama and Akiyoshi Takami and Yoshitaka Kodera",

note = "Publisher Copyright: {\textcopyright} 2022 American Society for Photobiology.",

year = "2022",

month = sep,

day = "1",

doi = "10.1111/php.13613",

language = "English",

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Maeda, A, Yamamoto, R, Mizuno, S, Miki, S, Sakamoto, Y, Kogata, S, Toyama, C, Sato, K, Okamatsu, C, Ando, T, Iida, M, Watsuji, T, Sato, T, Miyagawa, S, Okuyama, H, Takami, A & Kodera, Y 2022, 'Efficacy of a 365 nm Ultraviolet A1 light Emitting Diode (UVA1-LED) in in vitro Extracorporeal Photopheresis', Photochemistry and Photobiology, vol. 98, no. 5, pp. 1229-1235. https://doi.org/10.1111/php.13613

TY - JOUR

T1 - Efficacy of a 365 nm Ultraviolet A1 light Emitting Diode (UVA1-LED) in in vitro Extracorporeal Photopheresis

AU - Maeda, Akira

AU - Yamamoto, Riho

AU - Mizuno, Shohei

AU - Miki, Shigeichiro

AU - Sakamoto, Yasuhiro

AU - Kogata, Shuhei

AU - Toyama, Chiyoshi

AU - Sato, Kazuki

AU - Okamatsu, Chizu

AU - Ando, Takanori

AU - Iida, Minako

AU - Watsuji, Toru

AU - Sato, Toshinobu

AU - Miyagawa, Shuji

AU - Okuyama, Hiroomi

AU - Takami, Akiyoshi

AU - Kodera, Yoshitaka

PY - 2022/9/1

Y1 - 2022/9/1

N2 - Extracorporeal photochemotherapy (ECP) is one of the more effective cell therapies for graft-versus-host disease (GvHD). ECP is a widely recommended therapeutic approach for the treatment of chronic GvHD, particularly steroid-refractory GVHD. In recent years, the use of a light emitting diode (LED) in the clinic has attracted considerable interest. In this study, we examined the issue of whether an ultraviolet A1-light emitting diode (UVA1-LED) can be used as a light source in ECP. To compare the efficacy of ECP with conventional UVA lamp and a UVA1-LED, we established an in vitro ECP model. Treatment efficacy was evaluated by measuring the % apoptosis and the inhibition of T-cell proliferation. To investigate the effect of ECP on the innate immune reaction, THP-1 cells with a luciferase reporter gene driven by a NF-kB response element (THP-1 luc NF-kB) were treated with ECP. The LED-ECP induced apoptosis and inhibition of T-cell proliferation as efficiently as a conventional ECP. However, LED-ECP induced less innate immunity in THP-1. Since LED devices are more compact compared with conventional UVA irradiation devices, the use of a UVA1-LED in the treatment of ECP may be a better alternative to conventional ECP therapy.

AB - Extracorporeal photochemotherapy (ECP) is one of the more effective cell therapies for graft-versus-host disease (GvHD). ECP is a widely recommended therapeutic approach for the treatment of chronic GvHD, particularly steroid-refractory GVHD. In recent years, the use of a light emitting diode (LED) in the clinic has attracted considerable interest. In this study, we examined the issue of whether an ultraviolet A1-light emitting diode (UVA1-LED) can be used as a light source in ECP. To compare the efficacy of ECP with conventional UVA lamp and a UVA1-LED, we established an in vitro ECP model. Treatment efficacy was evaluated by measuring the % apoptosis and the inhibition of T-cell proliferation. To investigate the effect of ECP on the innate immune reaction, THP-1 cells with a luciferase reporter gene driven by a NF-kB response element (THP-1 luc NF-kB) were treated with ECP. The LED-ECP induced apoptosis and inhibition of T-cell proliferation as efficiently as a conventional ECP. However, LED-ECP induced less innate immunity in THP-1. Since LED devices are more compact compared with conventional UVA irradiation devices, the use of a UVA1-LED in the treatment of ECP may be a better alternative to conventional ECP therapy.

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U2 - 10.1111/php.13613

DO - 10.1111/php.13613

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AN - SCOPUS:85126023912

SN - 0031-8655

VL - 98

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JO - Photochemistry and Photobiology

JF - Photochemistry and Photobiology

IS - 5

ER -

Efficacy of a 365 nm Ultraviolet A1 light Emitting Diode (UVA1-LED) in in vitro Extracorporeal Photopheresis

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