Effects of water extract of Cajanus cajan leaves on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and the adipocytic trans-differentiation of mouse primary osteoblasts

Jinchao Zhang, Cuilian Liu, Jing Sun, Dandan Liu, Peng Wang

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The effects of water extract of Cajanus cajan (Linn.) Millsp. (Leguminosae) leaves (WECML) on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells (BMSCs) and the adipocytic trans-differentiation of mouse primary osteoblasts (OBs) were studied. The results indicated that WECML promoted the proliferation of BMSCs and OBs at most concentrations. WECML promoted the osteogenic differentiation and formation of mineralized matrix nodules of BMSCs at concentrations of 0.1, 1, and 10 μg/mL, but inhibited the osteogenic differentiation and formation of mineralized matrix nodules of BMSCs at concentration of 0.01 μg/mL. WECML inhibited the adipogenic differentiation of BMSCs and adipocytic trans-differentiation of OBs at concentrations of 0.001, 0.1, 1, 10, and 100 μg/mL, but had no effects at concentration of 0.01 μg/mL. The results suggest that WECML has protective effects on bone and these protective effects may be mediated by decreasing adipocytic cell formation from BMSCs, which may promote the proliferation, differentiation, and mineralization function of OBs. The defined active ingredients in the WECML and the active mechanism need to be further studied.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalPharmaceutical Biology
Volume48
Issue number1
DOIs
StatePublished - Jan 2010
Externally publishedYes

Keywords

  • Adipocytic trans-differentiation
  • Adipogenic differentiation
  • Bone marrow stromal cells
  • Cajanus cajan (Linn.) Millsp.
  • Osteogenic differentiation

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