TY - JOUR
T1 - Effects of lanthanum and gadolinium ions on cardiac sarcoplasmic reticulum
AU - Krasnow, Norman
N1 - Funding Information:
The author would like to thank Professor S. Ebashi and Dr Y. Ogawa for their helpful discussions; Mrs L. Shore and Mrs N. Lazarus for their excellent technical assistance; and Dr P. Dreizen for suggesting the use of gadolinium. This work was supported by Grant U-I638, Health Research Council of the City of New York and Grant HE-o5~26, U.S. Public Service, National Heart Institute, Bethesda, Md.
PY - 1972/9/1
Y1 - 1972/9/1
N2 - The tervalent rare earth cations lanthanum (La3+) and gadolinium (Gd3+) have been studied for their effect on canine cardiac sarcoplasmic reticulum. At dose-related concentrations above 10 μM, La3+ and Gd3+ inhibit calcium binding non-competitively, decrease the velocity of calcium uptake (in presence of oxalate), and inhibit the Ca2+-activated Mg2+-ATPase. The tendency for sarcoplasmic reticulum inhibition by excess Ca2+ is accentuated by lanthanides. The effect of the lanthanides is time-dependent, inhibition diminishing during incubation. Since effects were observed in the presence of oxalate, the reactive lanthanide ion concentration is not as predicted from solubility-product considerations.
AB - The tervalent rare earth cations lanthanum (La3+) and gadolinium (Gd3+) have been studied for their effect on canine cardiac sarcoplasmic reticulum. At dose-related concentrations above 10 μM, La3+ and Gd3+ inhibit calcium binding non-competitively, decrease the velocity of calcium uptake (in presence of oxalate), and inhibit the Ca2+-activated Mg2+-ATPase. The tendency for sarcoplasmic reticulum inhibition by excess Ca2+ is accentuated by lanthanides. The effect of the lanthanides is time-dependent, inhibition diminishing during incubation. Since effects were observed in the presence of oxalate, the reactive lanthanide ion concentration is not as predicted from solubility-product considerations.
UR - https://www.scopus.com/pages/publications/0015399453
U2 - 10.1016/0005-2736(72)90323-9
DO - 10.1016/0005-2736(72)90323-9
M3 - Article
C2 - 4262693
AN - SCOPUS:0015399453
SN - 0005-2736
VL - 282
SP - 187
EP - 194
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - C
ER -