Effects of influenza immunization on humoral and cellular alloreactivity in humans

Lara Danziger-Isakov, Leonid Cherkassky, Hanni Siegel, Mary McManamon, Kristen Kramer, Marie Budev, Deirdre Sawinski, Joshua J. Augustine, Donald E. Hricik, Robert Fairchild, Peter S. Heeger, Emilio D. Poggio

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


BACKGROUND.: Alloreactive T cells and anti-human leukocyte antigen antibodies mediate transplant injury. Environmental exposures, including vaccinations, may activate the alloimmune repertoire leading to accelerated allograft injury. To test whether vaccination impacts human alloimmunity, we analyzed humoral and cellular immune reactivity in subjects undergoing influenza vaccination. METHODS.: We serially obtained blood samples from 30 healthy subjects and 8 kidney and 9 lung transplant recipients who received influenza vaccination, and from 20 healthy unvaccinated controls. We measured cellular and humoral anti-influenza responses, anti-human leukocyte antigen antibodies, and alloreactive T-cell immunity (interferon-γ ELISPOT) at 0, 2, 4, and 12 weeks after vaccination. RESULTS.: Vaccination induced influenza-reactive humoral and cellular responses in control subjects and in transplant recipients. Only two of 30 vaccinated volunteers developed new alloantibodies, but none of the transplant patients. Vaccination also specifically and significantly augmented cellular alloimmunity based on reactivity to a panel of stimulators in both healthy subjects and in transplant recipients within 4 weeks of vaccination. The enhanced cellular alloresponse waned toward prevaccine levels by week 12. CONCLUSION.: Our findings newly demonstrate that influenza vaccination can have a significant impact on the potency of the alloimmune repertoire. Because the strength of the alloresponse influences long-term graft function, our results suggest that further investigation of alloimmune monitoring after vaccination is needed.

Original languageEnglish
Pages (from-to)838-844
Number of pages7
Issue number7
StatePublished - Apr 2010


  • Allosensitization
  • Heterologous immunity
  • Influenza
  • T cell
  • Vaccination


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