Effects of in vivo culture of murine mammary adenocarcinoma cells on tumor and metastatic growth

Julio A. Aguirre Ghiso, Miriam Diament, Isabel D’elia, Elisa Bal de Kier Joffé, Slobodanka Klein

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


We studied the effect of tumoral microenvironments on metastatic phenotypes. Therefore, murine mammary adenocarcinoma cells cultured in vivo in diffusion chambers (DC) were implanted intraperitoneally in BALB/c mice. The behavior of DC-cultured cells was compared with that of cells obtained from tumors growing subcutaneously or intraperitoneally and from primary cultures in vitro of the former. DC-cultured and control cells were inoculated into normal mice to evaluate their tumorigenicity and metastasizing ability. We found that DC-cultured cells were less tumorigenic and metastatic both in spontaneous and in experimental metastasis assays. The host reponse to tumor progression resulted in an early leukocytosis, probably due to the overproduction of a hematopoietic factor by the tumor cells. Finally, it was found that DC-cultured cells produced lower levels of urokinase-type plasmino-gen activator activity, while no differences were found in the metalloproteinase production compared to cells obtained from a tumor growing subcutaneously.

Original languageEnglish
Pages (from-to)41-52
Number of pages12
JournalTumor Biology
Issue number1
StatePublished - 1 Jan 1997
Externally publishedYes


  • Diffusion chambers
  • Hematological response
  • Metastasis
  • Microenvironment
  • Proteolytic enzymes
  • Tumor cells


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