Effects of analogs of 1,25(OH)2 Vitamin D3 on the proliferation and differentiation of the human chronic myelogenous leukemia cell line, RWLeu-4

Jeffrey W. Clark; Marshall R. Posner; Jean M. Marsella; Anabel Santos; Milan Uskokovic; Charles Eil; Stephen R. Lasky

doi:10.1007/BF01410133

Effects of analogs of 1,25(OH)₂ Vitamin D₃ on the proliferation and differentiation of the human chronic myelogenous leukemia cell line, RWLeu-4

Jeffrey W. Clark
, Marshall R. Posner
, Jean M. Marsella
, Anabel Santos
, Milan Uskokovic
, Charles Eil
, Stephen R. Lasky

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We evaluated the proliferative and differentiative effects of analogs of 1,25(OH)₂ vitamin D₃ [1,25(OH)₂D₃] on a chronic myelogenous leukemia cell line, RWLeu-4, which is growth-inhibited and differentiates in response to 1,25(OH)₂D₃ (ED₅₀-3-10 n M). Side-chain-fluorinated analogs were more potent (ED₅₀=0.7-2 n M) while most of those with altered saturation of the D ring or side-chain carbon-carbon bonds were equally or less effective than 1,25(OH)₂D₃. However, the two analogs with either two additional double bonds or an extra double and triple bond in the D ring had greater antiproliferatiive activity [1,25(OH)₂-16,23-diene D₃ (ED₅₀=2.7 n M) and 1,25(OH)₂-16-ene-23-yne D₃ (ED₅₀=0.7 n M)]. Since the latter of these has been reported to be less potent at mobilizing calcium than 1,25(OH)₂D₃, it (or a similar compound) may be a candidate for clinical use as an antineoplastic agent.

Original language	English
Pages (from-to)	190-194
Number of pages	5
Journal	Journal of Cancer Research and Clinical Oncology
Volume	118
Issue number	3
DOIs	https://doi.org/10.1007/BF01410133
State	Published - Mar 1992
Externally published	Yes

Keywords

Analogs
Chronic myelogenous leukemia cells
Differentiation
Proliferation
Vitamin D

Access to Document

10.1007/BF01410133

Cite this

@article{009c6a580f9d420bba66fe049bb4edfd,

title = "Effects of analogs of 1,25(OH)2 Vitamin D3 on the proliferation and differentiation of the human chronic myelogenous leukemia cell line, RWLeu-4",

abstract = "We evaluated the proliferative and differentiative effects of analogs of 1,25(OH)2 vitamin D3 [1,25(OH)2D3] on a chronic myelogenous leukemia cell line, RWLeu-4, which is growth-inhibited and differentiates in response to 1,25(OH)2D3 (ED50-3-10 n M). Side-chain-fluorinated analogs were more potent (ED50=0.7-2 n M) while most of those with altered saturation of the D ring or side-chain carbon-carbon bonds were equally or less effective than 1,25(OH)2D3. However, the two analogs with either two additional double bonds or an extra double and triple bond in the D ring had greater antiproliferatiive activity [1,25(OH)2-16,23-diene D3 (ED50=2.7 n M) and 1,25(OH)2-16-ene-23-yne D3 (ED50=0.7 n M)]. Since the latter of these has been reported to be less potent at mobilizing calcium than 1,25(OH)2D3, it (or a similar compound) may be a candidate for clinical use as an antineoplastic agent.",

keywords = "Analogs, Chronic myelogenous leukemia cells, Differentiation, Proliferation, Vitamin D",

author = "Clark, \{Jeffrey W.\} and Posner, \{Marshall R.\} and Marsella, \{Jean M.\} and Anabel Santos and Milan Uskokovic and Charles Eil and Lasky, \{Stephen R.\}",

year = "1992",

month = mar,

doi = "10.1007/BF01410133",

language = "English",

volume = "118",

pages = "190--194",

journal = "Journal of Cancer Research and Clinical Oncology",

issn = "0171-5216",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "3",

}

Effects of analogs of 1,25(OH)₂ Vitamin D₃ on the proliferation and differentiation of the human chronic myelogenous leukemia cell line, RWLeu-4. / Clark, Jeffrey W.; Posner, Marshall R.; Marsella, Jean M. et al.
In: Journal of Cancer Research and Clinical Oncology, Vol. 118, No. 3, 03.1992, p. 190-194.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Effects of analogs of 1,25(OH)2 Vitamin D3 on the proliferation and differentiation of the human chronic myelogenous leukemia cell line, RWLeu-4

AU - Clark, Jeffrey W.

AU - Posner, Marshall R.

AU - Marsella, Jean M.

AU - Santos, Anabel

AU - Uskokovic, Milan

AU - Eil, Charles

AU - Lasky, Stephen R.

PY - 1992/3

Y1 - 1992/3

N2 - We evaluated the proliferative and differentiative effects of analogs of 1,25(OH)2 vitamin D3 [1,25(OH)2D3] on a chronic myelogenous leukemia cell line, RWLeu-4, which is growth-inhibited and differentiates in response to 1,25(OH)2D3 (ED50-3-10 n M). Side-chain-fluorinated analogs were more potent (ED50=0.7-2 n M) while most of those with altered saturation of the D ring or side-chain carbon-carbon bonds were equally or less effective than 1,25(OH)2D3. However, the two analogs with either two additional double bonds or an extra double and triple bond in the D ring had greater antiproliferatiive activity [1,25(OH)2-16,23-diene D3 (ED50=2.7 n M) and 1,25(OH)2-16-ene-23-yne D3 (ED50=0.7 n M)]. Since the latter of these has been reported to be less potent at mobilizing calcium than 1,25(OH)2D3, it (or a similar compound) may be a candidate for clinical use as an antineoplastic agent.

AB - We evaluated the proliferative and differentiative effects of analogs of 1,25(OH)2 vitamin D3 [1,25(OH)2D3] on a chronic myelogenous leukemia cell line, RWLeu-4, which is growth-inhibited and differentiates in response to 1,25(OH)2D3 (ED50-3-10 n M). Side-chain-fluorinated analogs were more potent (ED50=0.7-2 n M) while most of those with altered saturation of the D ring or side-chain carbon-carbon bonds were equally or less effective than 1,25(OH)2D3. However, the two analogs with either two additional double bonds or an extra double and triple bond in the D ring had greater antiproliferatiive activity [1,25(OH)2-16,23-diene D3 (ED50=2.7 n M) and 1,25(OH)2-16-ene-23-yne D3 (ED50=0.7 n M)]. Since the latter of these has been reported to be less potent at mobilizing calcium than 1,25(OH)2D3, it (or a similar compound) may be a candidate for clinical use as an antineoplastic agent.

KW - Analogs

KW - Chronic myelogenous leukemia cells

KW - Differentiation

KW - Proliferation

KW - Vitamin D

UR - https://www.scopus.com/pages/publications/0026586118

U2 - 10.1007/BF01410133

DO - 10.1007/BF01410133

M3 - Article

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AN - SCOPUS:0026586118

SN - 0171-5216

VL - 118

SP - 190

EP - 194

JO - Journal of Cancer Research and Clinical Oncology

JF - Journal of Cancer Research and Clinical Oncology

IS - 3