TY - JOUR
T1 - Effects of amino acids on gelation kinetics and solubility of sickle hemoglobin
AU - Noguchi, C. T.
AU - Schechter, A. N.
N1 - Funding Information:
We thank Drs. W.A. Eaton, H.J. Hofrichter and P.D. Ross for many discussions; Drs. N.S. Young and P. Klug and Ms. M. Lloyd for blood sickle hemoglobin patients. C.T.N. is a recipient of a NIGMS Fellowship.
PY - 1977/1/24
Y1 - 1977/1/24
N2 - Ten amino acids have been studied for their effects on the gelation of sickle hemoglobin using the recently developed assay of Hofrichter, Ross and Eaton. By monitoring kinetics and using high speed sedimentation, the rate and extent of gelation are directly measured. Of the amino acids tested, only phenylalanine significantly inhibited the gelation of sickle hemoglobin. The systematic study of the effects of additives, such as amino acids, on gelation serves as a basis for the study of potential non-covalent inhibitors of sickling.
AB - Ten amino acids have been studied for their effects on the gelation of sickle hemoglobin using the recently developed assay of Hofrichter, Ross and Eaton. By monitoring kinetics and using high speed sedimentation, the rate and extent of gelation are directly measured. Of the amino acids tested, only phenylalanine significantly inhibited the gelation of sickle hemoglobin. The systematic study of the effects of additives, such as amino acids, on gelation serves as a basis for the study of potential non-covalent inhibitors of sickling.
UR - http://www.scopus.com/inward/record.url?scp=0017623401&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(77)90350-3
DO - 10.1016/0006-291X(77)90350-3
M3 - Article
C2 - 836316
AN - SCOPUS:0017623401
SN - 0006-291X
VL - 74
SP - 637
EP - 642
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -