Effective separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide by altering conditions of polyacrylamide gel electrophoresis

Jill P. Buyon; Seth G. Slade; Ed K.L. Chan; Eng M. Tan; Robert Winchester

doi:10.1016/0022-1759(90)90440-7

Effective separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide by altering conditions of polyacrylamide gel electrophoresis

Jill P. Buyon, Seth G. Slade, Ed K.L. Chan, Eng M. Tan, Robert Winchester

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Abstract

A method is described for the separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide. These two proteins anomalously co-migrate with the same relative mass under conditions of conventional sodium dodecyl sulfate polyacrylamide gel electrophoresis according to Laemmli using a stock solution of acrylamide: bisacrylamide 30:08, ratio = 37.5. A higher ratio of monomer to cross-linker, ratio = 172.4 used in a 15% acrylamide solution, readily separates the two peptide chains. This method facilitates the detection of the 52 kDa SSA/Ro component which otherwise might have been incorrectly assigned as a 48 kDa SSB/La polypeptide.

Original language	English
Pages (from-to)	207-210
Number of pages	4
Journal	Journal of Immunological Methods
Volume	129
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(90)90440-7
State	Published - 25 May 1990
Externally published	Yes

Keywords

Auto-antibody
SDS-PAGE
SSA/Ro, 52 kDa
SSB/La, 48 kDa

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10.1016/0022-1759(90)90440-7

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title = "Effective separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide by altering conditions of polyacrylamide gel electrophoresis",

abstract = "A method is described for the separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide. These two proteins anomalously co-migrate with the same relative mass under conditions of conventional sodium dodecyl sulfate polyacrylamide gel electrophoresis according to Laemmli using a stock solution of acrylamide: bisacrylamide 30:08, ratio = 37.5. A higher ratio of monomer to cross-linker, ratio = 172.4 used in a 15\% acrylamide solution, readily separates the two peptide chains. This method facilitates the detection of the 52 kDa SSA/Ro component which otherwise might have been incorrectly assigned as a 48 kDa SSB/La polypeptide.",

keywords = "Auto-antibody, SDS-PAGE, SSA/Ro, 52 kDa, SSB/La, 48 kDa",

author = "Buyon, \{Jill P.\} and Slade, \{Seth G.\} and Chan, \{Ed K.L.\} and Tan, \{Eng M.\} and Robert Winchester",

note = "Funding Information: This work was supported by a grant from the Lupus Foundation of America.",

year = "1990",

month = may,

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doi = "10.1016/0022-1759(90)90440-7",

language = "English",

volume = "129",

pages = "207--210",

journal = "Journal of Immunological Methods",

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publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Effective separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide by altering conditions of polyacrylamide gel electrophoresis

AU - Buyon, Jill P.

AU - Slade, Seth G.

AU - Chan, Ed K.L.

AU - Tan, Eng M.

AU - Winchester, Robert

N1 - Funding Information: This work was supported by a grant from the Lupus Foundation of America.

PY - 1990/5/25

Y1 - 1990/5/25

N2 - A method is described for the separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide. These two proteins anomalously co-migrate with the same relative mass under conditions of conventional sodium dodecyl sulfate polyacrylamide gel electrophoresis according to Laemmli using a stock solution of acrylamide: bisacrylamide 30:08, ratio = 37.5. A higher ratio of monomer to cross-linker, ratio = 172.4 used in a 15% acrylamide solution, readily separates the two peptide chains. This method facilitates the detection of the 52 kDa SSA/Ro component which otherwise might have been incorrectly assigned as a 48 kDa SSB/La polypeptide.

AB - A method is described for the separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide. These two proteins anomalously co-migrate with the same relative mass under conditions of conventional sodium dodecyl sulfate polyacrylamide gel electrophoresis according to Laemmli using a stock solution of acrylamide: bisacrylamide 30:08, ratio = 37.5. A higher ratio of monomer to cross-linker, ratio = 172.4 used in a 15% acrylamide solution, readily separates the two peptide chains. This method facilitates the detection of the 52 kDa SSA/Ro component which otherwise might have been incorrectly assigned as a 48 kDa SSB/La polypeptide.

KW - Auto-antibody

KW - SDS-PAGE

KW - SSA/Ro, 52 kDa

KW - SSB/La, 48 kDa

UR - https://www.scopus.com/pages/publications/0025194936

U2 - 10.1016/0022-1759(90)90440-7

DO - 10.1016/0022-1759(90)90440-7

M3 - Article

C2 - 2351837

AN - SCOPUS:0025194936

SN - 0022-1759

VL - 129

SP - 207

EP - 210

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 2

ER -

Effective separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide by altering conditions of polyacrylamide gel electrophoresis

Abstract

Keywords

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