Effect of tricomplex fixation on lung tissue

Joan Gil

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


The effect of tricomplex fixation on lungs fixed through vascular perfusion or through instillation of fixatives into the airways has been studied. To test the specificity of electron dense precipitates for phospholipids, some preparations were treated with tricomplex fixatives after lipid extraction with chloroform-methanol in a Soxhlet apparatus. The same experiment was repeated with red blood cells taken from the inferior vena cava of rats. It is concluded that precipitates reported in lung tissue after tricomplex flocculation cannot be regarded as specific for surfactant phospholipids, since they appear in various locations of several cell types including the interior of red blood cells; their occurrence is furthermore not influenced by lipid extraction prior to the application of tricomplex fixatives.

Original languageEnglish
Pages (from-to)122-131
Number of pages10
JournalJournal of Structural Biology
Issue number1-2
StatePublished - Jul 1972
Externally publishedYes


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