TY - JOUR
T1 - Effect of tricomplex fixation on lung tissue
AU - Gil, Joan
N1 - Funding Information:
Assistance was received from Miss G. Reber, Mrs R. Bachmann and Mr K. Babl. This work was supported by Grant No. 3.5.68 of the Swiss National Science Foundation.
PY - 1972/7
Y1 - 1972/7
N2 - The effect of tricomplex fixation on lungs fixed through vascular perfusion or through instillation of fixatives into the airways has been studied. To test the specificity of electron dense precipitates for phospholipids, some preparations were treated with tricomplex fixatives after lipid extraction with chloroform-methanol in a Soxhlet apparatus. The same experiment was repeated with red blood cells taken from the inferior vena cava of rats. It is concluded that precipitates reported in lung tissue after tricomplex flocculation cannot be regarded as specific for surfactant phospholipids, since they appear in various locations of several cell types including the interior of red blood cells; their occurrence is furthermore not influenced by lipid extraction prior to the application of tricomplex fixatives.
AB - The effect of tricomplex fixation on lungs fixed through vascular perfusion or through instillation of fixatives into the airways has been studied. To test the specificity of electron dense precipitates for phospholipids, some preparations were treated with tricomplex fixatives after lipid extraction with chloroform-methanol in a Soxhlet apparatus. The same experiment was repeated with red blood cells taken from the inferior vena cava of rats. It is concluded that precipitates reported in lung tissue after tricomplex flocculation cannot be regarded as specific for surfactant phospholipids, since they appear in various locations of several cell types including the interior of red blood cells; their occurrence is furthermore not influenced by lipid extraction prior to the application of tricomplex fixatives.
UR - http://www.scopus.com/inward/record.url?scp=0015368337&partnerID=8YFLogxK
U2 - 10.1016/S0022-5320(72)80026-1
DO - 10.1016/S0022-5320(72)80026-1
M3 - Article
C2 - 4556195
AN - SCOPUS:0015368337
SN - 0022-5320
VL - 40
SP - 122
EP - 131
JO - Journal of Structural Biology
JF - Journal of Structural Biology
IS - 1-2
ER -