Effect of stimulation with LPS on expression of high mobility group box1 in murine peritoneal macrophages

Shi Hong Yi, Lu Guo Sun, Cheng Guo Wei

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To observe the effect of stimulation with LPS on the expression of high mobility group boxl (HMGB1) in murine peritoneal macrophages. Methods: The peritoneal macrophages of normal mice were isolated and stimulated with LPS for 4, 8, 12, 18, 24 and 36 h respectively, then determined for transcription level of HMGB1 mRNA by RT-PCR and for expression level of HMGB1 protein by Western blot. The time-response relationship between LPS stimulation and HMGB1 release was observed. Results: The transcription level of HMGB1 mRNA showed no significant change 4-18 h, while increased significantly 24 h and showed significant difference with those of control 24 and 36 h after LPS stimulation. However, the expression level of HMGB1 protein increased significantly from 18 h until 36 h after LPS stimulation. Conclusion: LPS showed a time-dependent promoting effect on the expression of HMGB1 in murine peritoneal macrophages.

Original languageEnglish
Pages (from-to)768-770
Number of pages3
JournalChinese Journal of Biologicals
Volume22
Issue number8
StatePublished - Aug 2009
Externally publishedYes

Keywords

  • Expression
  • High mobility group box1 (HMGB1)
  • Lipopolysaccharide (LPS)
  • Macrophage

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