Effect of renal ischemia on cortical microsomal calcium accumulation

A. Schieppati, P. D. Wilson, T. J. Burke, R. W. Schrier

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13 Scopus citations


Mitochondrial respiration, Ca2+ content, and Ca2+ kinetics have been found to be profoundly altered in ischemic acute renal failure (ARF). The effect of clamping the bilateral renal artery for 50 and 90 min on microsomal Ca2+ uptake was therefore examined in the rat. The 50-min clamping produced a reversible model of nonoliguric ARF, and the 90-min clamping produced a model of nonreversible oliguric ARF. In the 50-min nonoliguric model, ATP-dependent Ca2+ uptake by microsomes from renal cortex (nmol·mg protein-1·30 min-1) was significantly impaired immediately before release of the clamp and before return of renal blood flow (reflow) (191 ± 11 vs. 83 ± 11, P < 0.005). However, in this nonoliguric model of ischemic ARF, microsomal uptake returned completely to normal after 1 h of reflow (sham 189 ± 11 vs. 167 ± 14 at 1 h, NS) and persisted at this normal level at 24 h (sham 166 ± 14 vs. 150 ± 13 at 24 h, NS). In the oliguric model of ARF the microsomal Ca2+ uptake also was impaired immediately after the clamp release (sham 191 ± 11 vs. 93 ± 11, P < 0.001) as well as after 1 h of reflow (sham 189 ± 11 vs. 129 ± 12, P < 0.005) but not at 24 h (sham 166 ± 14 vs. 173 ± 13, NS). The results indicate that impaired microsomal Ca2+ uptake occurs early in both oliguric and nonoliguric ARF and persists after 1 h of reflow in the oliguric model. However, after 24 h of reflow in models of ARF, no evidence of impaired microsomal Ca2+ uptake was observed.

Original languageEnglish
Pages (from-to)C476-C483
JournalAmerican Journal of Physiology - Cell Physiology
Issue number3
StatePublished - 1985
Externally publishedYes


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