Effect of raised extracellular calcium on cell spread area in quail medullary bone osteoclasts

ZA Bascal, AS Alam, M. Zaidi, CG Dacke

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The present study reports on the effects of extracellular calcium ([Ca2+]o) elevation and ionomycin on cell spread area of medullary bone osteoclasts freshly isolated from egg‐laying Japanese quail. The responses were compared with those demonstrated in osteoclasts cultured for periods of 5–8 days and also to those previously demonstrated in neonatal rat osteoclasts. Freshly isolated medullary bone osteoclasts, unlike rat osteoclasts, were refractory to 20 mM [Ca2+]o, in that they showed no change in cell spread area. They did, however, show a modest (15%) reduction in cell spread area to ionomycin (7–50 microM), applied for 15–30 min. When medullary bone osteoclasts were precultured for 5–8 days, they exhibited a well‐developed response to 20 mM [Ca2+]o with a 46% reduction in cell spread area. They also showed a similar reduction in cell spread area in response to ionomycin (4 microM). It is concluded that, unlike freshly isolated neonatal rat osteoclasts, those obtained from quail medullary bone appear refractory to inhibitory factors such as [Ca2+]o. However, when the avian cells are cultured for a few days they appear to recover their ability to respond to [Ca2+]o.

Original languageEnglish
Pages (from-to)15-24
Number of pages10
JournalExperimental Physiology
Volume79
Issue number1
DOIs
StatePublished - 1 Jan 1994

Fingerprint

Dive into the research topics of 'Effect of raised extracellular calcium on cell spread area in quail medullary bone osteoclasts'. Together they form a unique fingerprint.

Cite this