Effect of prostaglandin E2 on gamma-interferon and 1,25(OH)2D3 vitamin D3-induced c-myc reduction during HL-60 cell differentiation

Toshimitsu Matsui; Yoshinobu Nakao; Tamio Koizumi; Yuko Katakami; Rei Takahashi; Kouchiro Mihara; Taketoshi Sugiyama; Takuo Fujita

doi:10.1016/0145-2126(88)90090-2

Effect of prostaglandin E₂ on gamma-interferon and 1,25(OH)₂D₃ vitamin D₃-induced c-myc reduction during HL-60 cell differentiation

Toshimitsu Matsui, Yoshinobu Nakao, Tamio Koizumi, Yuko Katakami, Rei Takahashi, Kouchiro Mihara, Taketoshi Sugiyama, Takuo Fujita

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The effect of prostaglandin E₂ (PGE₂) on the reduction of c-myc expression during the differentiation of the human leukemic cell line, HL-60, was examined. PGE₂, a potent inducer of intracellular cyclic AMP (cAMP) in HL-60 cells, augmented monocyte-associated cell surface antigens induced by human gamma-interferon (IFN-γ) or 1α,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃) in these cells. The elevation of intracellular cAMP was induced dose-dependently by PGE₂, but not by IFN-γ or 1,25(OH)₂D₃. Changes were also seen in functional differentiation, such as, the increase of phagocytic capability and superoxide generation. PGE₂ also enhanced the reduction of c-myc expression and the down-regulation of transferrin receptor by IFN-γ or 1,25(OH)₂D₃, whereas PGE₂ alone did not induce these phenotypic changes. These data suggest that IFN-γ and 1,25(OH)₂D₃ reduce c-myc expression of HL-60 cells by a mechanism other than the augmentation of intracellular cAMP.

Original language	English
Pages (from-to)	597-605
Number of pages	9
Journal	Leukemia Research
Volume	12
Issue number	7
DOIs	https://doi.org/10.1016/0145-2126(88)90090-2
State	Published - 1988
Externally published	Yes

Keywords

1
25(OH)D
Differentiation
PGE IFN-γ
c-myc

Access to Document

10.1016/0145-2126(88)90090-2

Cite this

@article{eed087ddc1724a87b207570c765483cb,

title = "Effect of prostaglandin E2 on gamma-interferon and 1,25(OH)2D3 vitamin D3-induced c-myc reduction during HL-60 cell differentiation",

abstract = "The effect of prostaglandin E2 (PGE2) on the reduction of c-myc expression during the differentiation of the human leukemic cell line, HL-60, was examined. PGE2, a potent inducer of intracellular cyclic AMP (cAMP) in HL-60 cells, augmented monocyte-associated cell surface antigens induced by human gamma-interferon (IFN-γ) or 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) in these cells. The elevation of intracellular cAMP was induced dose-dependently by PGE2, but not by IFN-γ or 1,25(OH)2D3. Changes were also seen in functional differentiation, such as, the increase of phagocytic capability and superoxide generation. PGE2 also enhanced the reduction of c-myc expression and the down-regulation of transferrin receptor by IFN-γ or 1,25(OH)2D3, whereas PGE2 alone did not induce these phenotypic changes. These data suggest that IFN-γ and 1,25(OH)2D3 reduce c-myc expression of HL-60 cells by a mechanism other than the augmentation of intracellular cAMP.",

keywords = "1, 25(OH)D, Differentiation, PGE IFN-γ, c-myc",

author = "Toshimitsu Matsui and Yoshinobu Nakao and Tamio Koizumi and Yuko Katakami and Rei Takahashi and Kouchiro Mihara and Taketoshi Sugiyama and Takuo Fujita",

note = "Funding Information: Acknowledgements--We are most grateful to Dr Robert C. Gallo, National Cancer Institute, NIH, Bethesda for providing HL-60 cells and the DNA probe of c-myc. We thank Mr D. Mrozek for his critical reading of the manuscript. This work was supported in part by a Grant-in-Aid for cancer research from the Ministry of Health and Welfare of Japan (59S-1) and from the Ministry of Education, Science and Culture (62614523).",

year = "1988",

doi = "10.1016/0145-2126(88)90090-2",

language = "English",

volume = "12",

pages = "597--605",

journal = "Leukemia Research",

issn = "0145-2126",

publisher = "Elsevier Ltd.",

number = "7",

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TY - JOUR

T1 - Effect of prostaglandin E2 on gamma-interferon and 1,25(OH)2D3 vitamin D3-induced c-myc reduction during HL-60 cell differentiation

AU - Matsui, Toshimitsu

AU - Nakao, Yoshinobu

AU - Koizumi, Tamio

AU - Katakami, Yuko

AU - Takahashi, Rei

AU - Mihara, Kouchiro

AU - Sugiyama, Taketoshi

AU - Fujita, Takuo

N1 - Funding Information: Acknowledgements--We are most grateful to Dr Robert C. Gallo, National Cancer Institute, NIH, Bethesda for providing HL-60 cells and the DNA probe of c-myc. We thank Mr D. Mrozek for his critical reading of the manuscript. This work was supported in part by a Grant-in-Aid for cancer research from the Ministry of Health and Welfare of Japan (59S-1) and from the Ministry of Education, Science and Culture (62614523).

PY - 1988

Y1 - 1988

N2 - The effect of prostaglandin E2 (PGE2) on the reduction of c-myc expression during the differentiation of the human leukemic cell line, HL-60, was examined. PGE2, a potent inducer of intracellular cyclic AMP (cAMP) in HL-60 cells, augmented monocyte-associated cell surface antigens induced by human gamma-interferon (IFN-γ) or 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) in these cells. The elevation of intracellular cAMP was induced dose-dependently by PGE2, but not by IFN-γ or 1,25(OH)2D3. Changes were also seen in functional differentiation, such as, the increase of phagocytic capability and superoxide generation. PGE2 also enhanced the reduction of c-myc expression and the down-regulation of transferrin receptor by IFN-γ or 1,25(OH)2D3, whereas PGE2 alone did not induce these phenotypic changes. These data suggest that IFN-γ and 1,25(OH)2D3 reduce c-myc expression of HL-60 cells by a mechanism other than the augmentation of intracellular cAMP.

AB - The effect of prostaglandin E2 (PGE2) on the reduction of c-myc expression during the differentiation of the human leukemic cell line, HL-60, was examined. PGE2, a potent inducer of intracellular cyclic AMP (cAMP) in HL-60 cells, augmented monocyte-associated cell surface antigens induced by human gamma-interferon (IFN-γ) or 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) in these cells. The elevation of intracellular cAMP was induced dose-dependently by PGE2, but not by IFN-γ or 1,25(OH)2D3. Changes were also seen in functional differentiation, such as, the increase of phagocytic capability and superoxide generation. PGE2 also enhanced the reduction of c-myc expression and the down-regulation of transferrin receptor by IFN-γ or 1,25(OH)2D3, whereas PGE2 alone did not induce these phenotypic changes. These data suggest that IFN-γ and 1,25(OH)2D3 reduce c-myc expression of HL-60 cells by a mechanism other than the augmentation of intracellular cAMP.

KW - 1

KW - 25(OH)D

KW - Differentiation

KW - PGE IFN-γ

KW - c-myc

UR - https://www.scopus.com/pages/publications/0023821378

U2 - 10.1016/0145-2126(88)90090-2

DO - 10.1016/0145-2126(88)90090-2

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AN - SCOPUS:0023821378

SN - 0145-2126

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