TY - JOUR
T1 - Effect of pre-harvest application of calcium and boron on dietary fibre, hydrolases and ultrastructure in 'Cara Cara' navel orange (Citrus sinensis L. Osbeck) fruit
AU - Dong, Tao
AU - Xia, Renxue
AU - Xiao, Zhiyan
AU - Wang, Peng
AU - Song, Wenhua
N1 - Funding Information:
This work was supported by science and technology exploitation special item (2004EP090019) for Three-Gorges migrant, Ministry of Science and Technology Department of the People's Republic of China. The authors would like to thank Prof. Jihong Liu for his suggestions.
PY - 2009/7/2
Y1 - 2009/7/2
N2 - High dietary fibre diets are associated with the prevention, reduction and treatment of some diseases, such as diverticular and coronary heart diseases. However, pre-harvest calcium and boron sprays associated with the dietary fibre compounds in fruits remain unknown. In the present study, specific activities and gene expression levels of hydrolases were determined in order to identify the effect of pre-harvest application of Ca and B alone or in combination on dietary fibre and the ultrastructure of navel orange fruit (Citrus sinenisis L. Osbeck). The results showed that pre-harvest application of Ca, B had significant effect on the cross-linked polymer network of fruit segment membrane. In accordance with the enzyme activities, expression levels of polygalacturonase, pectinesterase and β-galactosidase were significantly reduced by pre-harvest application of Ca, B alone or in combination. Such treatments increased contents of total dietary fibre, insoluble dietary fibre, protopectin and cellulose, decreased soluble dietary fibre and water soluble pectin. Our study indicated that pre-harvest foliar application of Ca, B is useful for improving the tissue structure of segment membrane, reducing transcript levels and activities of polygalacturonase, pectinesterase and β-galactosidase and regulating the content of dietary fibre on Cara Cara navel orange.
AB - High dietary fibre diets are associated with the prevention, reduction and treatment of some diseases, such as diverticular and coronary heart diseases. However, pre-harvest calcium and boron sprays associated with the dietary fibre compounds in fruits remain unknown. In the present study, specific activities and gene expression levels of hydrolases were determined in order to identify the effect of pre-harvest application of Ca and B alone or in combination on dietary fibre and the ultrastructure of navel orange fruit (Citrus sinenisis L. Osbeck). The results showed that pre-harvest application of Ca, B had significant effect on the cross-linked polymer network of fruit segment membrane. In accordance with the enzyme activities, expression levels of polygalacturonase, pectinesterase and β-galactosidase were significantly reduced by pre-harvest application of Ca, B alone or in combination. Such treatments increased contents of total dietary fibre, insoluble dietary fibre, protopectin and cellulose, decreased soluble dietary fibre and water soluble pectin. Our study indicated that pre-harvest foliar application of Ca, B is useful for improving the tissue structure of segment membrane, reducing transcript levels and activities of polygalacturonase, pectinesterase and β-galactosidase and regulating the content of dietary fibre on Cara Cara navel orange.
KW - Calcium and boron
KW - Cara Cara navel orange
KW - Dietary fibre
KW - Real-time PCR
KW - Ultrastructure
UR - http://www.scopus.com/inward/record.url?scp=67349227768&partnerID=8YFLogxK
U2 - 10.1016/j.scienta.2009.02.003
DO - 10.1016/j.scienta.2009.02.003
M3 - Article
AN - SCOPUS:67349227768
SN - 0304-4238
VL - 121
SP - 272
EP - 277
JO - Scientia Horticulturae
JF - Scientia Horticulturae
IS - 3
ER -