Effect of Leflunomide and Cidofovir on replication of BK virus in an in vitro culture system

Noush Afarin Farasati; Ron Shapiro; Abhay Vats; Parmjeet Randhawa

doi:10.1097/01.TP.0000149338.97084.5F

Effect of Leflunomide and Cidofovir on replication of BK virus in an in vitro culture system

Noush Afarin Farasati, Ron Shapiro, Abhay Vats, Parmjeet Randhawa

Research output: Contribution to journal › Article › peer-review

139 Scopus citations

Abstract

Background. Cidofovir and Leflunomide are used empirically in the treatment of BK virus nephropathy. The aim of this study is to quantify the antiviral activity of these drugs. Methods. BK virus was grown in a cell-culture system. The rate of viral replication in the presence or absence of the drug being tested was assessed using a quantitative polymerase chain reaction assay. Results. The inhibitory concentration, effective concentration, and selectivity index for Leflunomide are 39.7±6.9, 11.3±2.8, and 3.8±0.8 μg/mL, respectively. For Cidofovir, these indices were, respectively, 63.9±17.2, 36.3±11.7, and 2.3±0.8 μg/mL. Conclusions. The in vitro activity of Cidofovir and Leflunomide is modest, and the selectivity index is low. There is a need to develop more effective and less toxic anti-BK virus drugs for clinical use.

Original language	English
Pages (from-to)	116-118
Number of pages	3
Journal	Transplantation
Volume	79
Issue number	1
DOIs	https://doi.org/10.1097/01.TP.0000149338.97084.5F
State	Published - 15 Jan 2005
Externally published	Yes

Keywords

BK virus
Cidofovir
Culture
Leflunomid

Access to Document

10.1097/01.TP.0000149338.97084.5F

Cite this

@article{64dd5511ef6a47aab97808dfc52139c7,

title = "Effect of Leflunomide and Cidofovir on replication of BK virus in an in vitro culture system",

abstract = "Background. Cidofovir and Leflunomide are used empirically in the treatment of BK virus nephropathy. The aim of this study is to quantify the antiviral activity of these drugs. Methods. BK virus was grown in a cell-culture system. The rate of viral replication in the presence or absence of the drug being tested was assessed using a quantitative polymerase chain reaction assay. Results. The inhibitory concentration, effective concentration, and selectivity index for Leflunomide are 39.7±6.9, 11.3±2.8, and 3.8±0.8 μg/mL, respectively. For Cidofovir, these indices were, respectively, 63.9±17.2, 36.3±11.7, and 2.3±0.8 μg/mL. Conclusions. The in vitro activity of Cidofovir and Leflunomide is modest, and the selectivity index is low. There is a need to develop more effective and less toxic anti-BK virus drugs for clinical use.",

keywords = "BK virus, Cidofovir, Culture, Leflunomid",

author = "Farasati, {Noush Afarin} and Ron Shapiro and Abhay Vats and Parmjeet Randhawa",

year = "2005",

month = jan,

day = "15",

doi = "10.1097/01.TP.0000149338.97084.5F",

language = "English",

volume = "79",

pages = "116--118",

journal = "Transplantation",

issn = "0041-1337",

publisher = "Lippincott Williams and Wilkins",

number = "1",

}

TY - JOUR

T1 - Effect of Leflunomide and Cidofovir on replication of BK virus in an in vitro culture system

AU - Farasati, Noush Afarin

AU - Shapiro, Ron

AU - Vats, Abhay

AU - Randhawa, Parmjeet

PY - 2005/1/15

Y1 - 2005/1/15

N2 - Background. Cidofovir and Leflunomide are used empirically in the treatment of BK virus nephropathy. The aim of this study is to quantify the antiviral activity of these drugs. Methods. BK virus was grown in a cell-culture system. The rate of viral replication in the presence or absence of the drug being tested was assessed using a quantitative polymerase chain reaction assay. Results. The inhibitory concentration, effective concentration, and selectivity index for Leflunomide are 39.7±6.9, 11.3±2.8, and 3.8±0.8 μg/mL, respectively. For Cidofovir, these indices were, respectively, 63.9±17.2, 36.3±11.7, and 2.3±0.8 μg/mL. Conclusions. The in vitro activity of Cidofovir and Leflunomide is modest, and the selectivity index is low. There is a need to develop more effective and less toxic anti-BK virus drugs for clinical use.

AB - Background. Cidofovir and Leflunomide are used empirically in the treatment of BK virus nephropathy. The aim of this study is to quantify the antiviral activity of these drugs. Methods. BK virus was grown in a cell-culture system. The rate of viral replication in the presence or absence of the drug being tested was assessed using a quantitative polymerase chain reaction assay. Results. The inhibitory concentration, effective concentration, and selectivity index for Leflunomide are 39.7±6.9, 11.3±2.8, and 3.8±0.8 μg/mL, respectively. For Cidofovir, these indices were, respectively, 63.9±17.2, 36.3±11.7, and 2.3±0.8 μg/mL. Conclusions. The in vitro activity of Cidofovir and Leflunomide is modest, and the selectivity index is low. There is a need to develop more effective and less toxic anti-BK virus drugs for clinical use.

KW - BK virus

KW - Cidofovir

KW - Culture

KW - Leflunomid

UR - http://www.scopus.com/inward/record.url?scp=12344300330&partnerID=8YFLogxK

U2 - 10.1097/01.TP.0000149338.97084.5F

DO - 10.1097/01.TP.0000149338.97084.5F

M3 - Article

C2 - 15714178

AN - SCOPUS:12344300330

SN - 0041-1337

VL - 79

SP - 116

EP - 118

JO - Transplantation

JF - Transplantation

IS - 1

ER -

Effect of Leflunomide and Cidofovir on replication of BK virus in an in vitro culture system

Abstract

Keywords

Access to Document

Fingerprint

Cite this