Effect of high density lipoproteins on protein expression in myoblast cell lines

Nikolina Babic; Craig Beeson; Norman J. Dovichi

doi:10.1021/pr049826g

Effect of high density lipoproteins on protein expression in myoblast cell lines

Nikolina Babic
, Craig Beeson
, Norman J. Dovichi

Research output: Contribution to journal › Article › peer-review

Abstract

We have studied the effects of high-density lipoprotein (HDL) on rat heart (H9c2) and skeletal (L6) myoblasts using two-dimensional gel electrophoresis and tandem mass spectrometry. Gels generated from control cells and cells treated with 250 μg/mL HDL showed significant differences in the 7-10 p/ region and the 30-50 kDa mass region. In particular, the membrane binding protein, annexin II, the voltage-dependent anion channel, glyceraldehyde-3-phosphate dehydrogenase and other glycolytic proteins are differentially expressed.

Original language	English
Pages (from-to)	344-348
Number of pages	5
Journal	Journal of Proteome Research
Volume	4
Issue number	2
DOIs	https://doi.org/10.1021/pr049826g
State	Published - Mar 2005
Externally published	Yes

Keywords

2-D electrophoresis
High-density lipoprotein
Myoblast

Access to Document

10.1021/pr049826g

Cite this

@article{62075ad96c7844f28c222342ba0e5917,

title = "Effect of high density lipoproteins on protein expression in myoblast cell lines",

abstract = "We have studied the effects of high-density lipoprotein (HDL) on rat heart (H9c2) and skeletal (L6) myoblasts using two-dimensional gel electrophoresis and tandem mass spectrometry. Gels generated from control cells and cells treated with 250 μg/mL HDL showed significant differences in the 7-10 p/ region and the 30-50 kDa mass region. In particular, the membrane binding protein, annexin II, the voltage-dependent anion channel, glyceraldehyde-3-phosphate dehydrogenase and other glycolytic proteins are differentially expressed.",

keywords = "2-D electrophoresis, High-density lipoprotein, Myoblast",

author = "Nikolina Babic and Craig Beeson and Dovichi, \{Norman J.\}",

year = "2005",

month = mar,

doi = "10.1021/pr049826g",

language = "English",

volume = "4",

pages = "344--348",

journal = "Journal of Proteome Research",

issn = "1535-3893",

publisher = "American Chemical Society",

number = "2",

}

TY - JOUR

T1 - Effect of high density lipoproteins on protein expression in myoblast cell lines

AU - Babic, Nikolina

AU - Beeson, Craig

AU - Dovichi, Norman J.

PY - 2005/3

Y1 - 2005/3

N2 - We have studied the effects of high-density lipoprotein (HDL) on rat heart (H9c2) and skeletal (L6) myoblasts using two-dimensional gel electrophoresis and tandem mass spectrometry. Gels generated from control cells and cells treated with 250 μg/mL HDL showed significant differences in the 7-10 p/ region and the 30-50 kDa mass region. In particular, the membrane binding protein, annexin II, the voltage-dependent anion channel, glyceraldehyde-3-phosphate dehydrogenase and other glycolytic proteins are differentially expressed.

AB - We have studied the effects of high-density lipoprotein (HDL) on rat heart (H9c2) and skeletal (L6) myoblasts using two-dimensional gel electrophoresis and tandem mass spectrometry. Gels generated from control cells and cells treated with 250 μg/mL HDL showed significant differences in the 7-10 p/ region and the 30-50 kDa mass region. In particular, the membrane binding protein, annexin II, the voltage-dependent anion channel, glyceraldehyde-3-phosphate dehydrogenase and other glycolytic proteins are differentially expressed.

KW - 2-D electrophoresis

KW - High-density lipoprotein

KW - Myoblast

UR - https://www.scopus.com/pages/publications/17444430421

U2 - 10.1021/pr049826g

DO - 10.1021/pr049826g

M3 - Article

C2 - 15822909

AN - SCOPUS:17444430421

SN - 1535-3893

VL - 4

SP - 344

EP - 348

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 2

ER -

Effect of high density lipoproteins on protein expression in myoblast cell lines

Abstract

Keywords

Access to Document

Fingerprint

Cite this