Abstract
We have studied the effects of high-density lipoprotein (HDL) on rat heart (H9c2) and skeletal (L6) myoblasts using two-dimensional gel electrophoresis and tandem mass spectrometry. Gels generated from control cells and cells treated with 250 μg/mL HDL showed significant differences in the 7-10 p/ region and the 30-50 kDa mass region. In particular, the membrane binding protein, annexin II, the voltage-dependent anion channel, glyceraldehyde-3-phosphate dehydrogenase and other glycolytic proteins are differentially expressed.
Original language | English |
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Pages (from-to) | 344-348 |
Number of pages | 5 |
Journal | Journal of Proteome Research |
Volume | 4 |
Issue number | 2 |
DOIs | |
State | Published - Mar 2005 |
Externally published | Yes |
Keywords
- 2-D electrophoresis
- High-density lipoprotein
- Myoblast