Effect of high density lipoproteins on protein expression in myoblast cell lines

Nikolina Babic, Craig Beeson, Norman J. Dovichi

Research output: Contribution to journalArticlepeer-review

Abstract

We have studied the effects of high-density lipoprotein (HDL) on rat heart (H9c2) and skeletal (L6) myoblasts using two-dimensional gel electrophoresis and tandem mass spectrometry. Gels generated from control cells and cells treated with 250 μg/mL HDL showed significant differences in the 7-10 p/ region and the 30-50 kDa mass region. In particular, the membrane binding protein, annexin II, the voltage-dependent anion channel, glyceraldehyde-3-phosphate dehydrogenase and other glycolytic proteins are differentially expressed.

Original languageEnglish
Pages (from-to)344-348
Number of pages5
JournalJournal of Proteome Research
Volume4
Issue number2
DOIs
StatePublished - Mar 2005
Externally publishedYes

Keywords

  • 2-D electrophoresis
  • High-density lipoprotein
  • Myoblast

Fingerprint

Dive into the research topics of 'Effect of high density lipoproteins on protein expression in myoblast cell lines'. Together they form a unique fingerprint.

Cite this