Effect of high blood flow and low mixed venous PO2 on oxygenation in isolated perfused rabbit lungs

I. Ayappa; L. V. Brown; J. S. Evans; S. J. Lai-Fook

Effect of high blood flow and low mixed venous PO₂ on oxygenation in isolated perfused rabbit lungs

I. Ayappa, L. V. Brown, J. S. Evans, S. J. Lai-Fook

Research output: Contribution to journal › Article › peer-review

Abstract

Capillary transit time measured directly in isolated rabbit lungs by fluorescence videomicroscopy decreased from 0.6 s to 0.15 s as blood flow increased 3-fold from 80 ml. min^-1, kg^-1 (control) (FASEB J. 9: A275,1995). Here, transit time was measured indirectly by dividing capillary blood volume (CO diffusing capacity method) by flow. Capillary transit time decreased from 0.5 s to 0.18 s as flow increased to 3-6 times control, confirming direct method. To study the effect of these short transit times on O₂ saturation, we perfused two lungs in series. Blood from one lung was O₂ desaturated by ventilating with 10% CO₂-90% N₂, and saturated by the test lung ventilated with air. Previous results showed 100% saturation at 3 times control flows with "mixed venous" PO₂ (PvO₂) to test lung of 40 mmHg. Here we decreased P_vO₂ to ̃20 mmHg, as observed during exercise. Table summarizes results [mean ±SD (n)]: Flow P_VO₂ P_a0₂ A-a DO₂ ΔO₂ Sat. 80 (5) 24 ± 8 125±14 -5 ±10 60% 240 (4) 2213 126 ±10 -6±8 70% O₂ saturation was 100% with no significant alveolar gas-to-end capillary blood O₂ gradient (A-a DO₂). No transit time limitation 'was detected. Supported by HL 36597 and HL 36552.

Original language	English
Pages (from-to)	A102
Journal	FASEB Journal
Volume	10
Issue number	3
State	Published - 1996
Externally published	Yes

Cite this

@article{48559e9b4079401eacb4c016a61f5bf6,

title = "Effect of high blood flow and low mixed venous PO2 on oxygenation in isolated perfused rabbit lungs",

abstract = "Capillary transit time measured directly in isolated rabbit lungs by fluorescence videomicroscopy decreased from 0.6 s to 0.15 s as blood flow increased 3-fold from 80 ml. min-1, kg-1 (control) (FASEB J. 9: A275,1995). Here, transit time was measured indirectly by dividing capillary blood volume (CO diffusing capacity method) by flow. Capillary transit time decreased from 0.5 s to 0.18 s as flow increased to 3-6 times control, confirming direct method. To study the effect of these short transit times on O2 saturation, we perfused two lungs in series. Blood from one lung was O2 desaturated by ventilating with 10% CO2-90% N2, and saturated by the test lung ventilated with air. Previous results showed 100% saturation at 3 times control flows with {"}mixed venous{"} PO2 (PvO2) to test lung of 40 mmHg. Here we decreased PvO2 to{\~ }20 mmHg, as observed during exercise. Table summarizes results [mean ±SD (n)]: Flow PVO2 Pa02 A-a DO2 ΔO2 Sat. 80 (5) 24 ± 8 125±14 -5 ±10 60% 240 (4) 2213 126 ±10 -6±8 70% O2 saturation was 100% with no significant alveolar gas-to-end capillary blood O2 gradient (A-a DO2). No transit time limitation 'was detected. Supported by HL 36597 and HL 36552.",

author = "I. Ayappa and Brown, {L. V.} and Evans, {J. S.} and Lai-Fook, {S. J.}",

year = "1996",

language = "English",

volume = "10",

pages = "A102",

journal = "FASEB Journal",

issn = "0892-6638",

publisher = "John Wiley & Sons Inc.",

number = "3",

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TY - JOUR

T1 - Effect of high blood flow and low mixed venous PO2 on oxygenation in isolated perfused rabbit lungs

AU - Ayappa, I.

AU - Brown, L. V.

AU - Evans, J. S.

AU - Lai-Fook, S. J.

PY - 1996

Y1 - 1996

N2 - Capillary transit time measured directly in isolated rabbit lungs by fluorescence videomicroscopy decreased from 0.6 s to 0.15 s as blood flow increased 3-fold from 80 ml. min-1, kg-1 (control) (FASEB J. 9: A275,1995). Here, transit time was measured indirectly by dividing capillary blood volume (CO diffusing capacity method) by flow. Capillary transit time decreased from 0.5 s to 0.18 s as flow increased to 3-6 times control, confirming direct method. To study the effect of these short transit times on O2 saturation, we perfused two lungs in series. Blood from one lung was O2 desaturated by ventilating with 10% CO2-90% N2, and saturated by the test lung ventilated with air. Previous results showed 100% saturation at 3 times control flows with "mixed venous" PO2 (PvO2) to test lung of 40 mmHg. Here we decreased PvO2 to ̃20 mmHg, as observed during exercise. Table summarizes results [mean ±SD (n)]: Flow PVO2 Pa02 A-a DO2 ΔO2 Sat. 80 (5) 24 ± 8 125±14 -5 ±10 60% 240 (4) 2213 126 ±10 -6±8 70% O2 saturation was 100% with no significant alveolar gas-to-end capillary blood O2 gradient (A-a DO2). No transit time limitation 'was detected. Supported by HL 36597 and HL 36552.

AB - Capillary transit time measured directly in isolated rabbit lungs by fluorescence videomicroscopy decreased from 0.6 s to 0.15 s as blood flow increased 3-fold from 80 ml. min-1, kg-1 (control) (FASEB J. 9: A275,1995). Here, transit time was measured indirectly by dividing capillary blood volume (CO diffusing capacity method) by flow. Capillary transit time decreased from 0.5 s to 0.18 s as flow increased to 3-6 times control, confirming direct method. To study the effect of these short transit times on O2 saturation, we perfused two lungs in series. Blood from one lung was O2 desaturated by ventilating with 10% CO2-90% N2, and saturated by the test lung ventilated with air. Previous results showed 100% saturation at 3 times control flows with "mixed venous" PO2 (PvO2) to test lung of 40 mmHg. Here we decreased PvO2 to ̃20 mmHg, as observed during exercise. Table summarizes results [mean ±SD (n)]: Flow PVO2 Pa02 A-a DO2 ΔO2 Sat. 80 (5) 24 ± 8 125±14 -5 ±10 60% 240 (4) 2213 126 ±10 -6±8 70% O2 saturation was 100% with no significant alveolar gas-to-end capillary blood O2 gradient (A-a DO2). No transit time limitation 'was detected. Supported by HL 36597 and HL 36552.

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M3 - Article

AN - SCOPUS:33748985838

SN - 0892-6638

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JO - FASEB Journal

JF - FASEB Journal

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ER -

Effect of high blood flow and low mixed venous PO2 on oxygenation in isolated perfused rabbit lungs

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Effect of high blood flow and low mixed venous PO₂ on oxygenation in isolated perfused rabbit lungs