Effect of eliminase gene (elmA) deletion on heparosan production and shedding in Escherichia coli K5

Anne Marie Hickey, Ujjwal Bhaskar, Robert J. Linhardt, Jonathan S. Dordick

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Escherichia coli K5 produces heparosan and sheds it into the growth medium in a temperature dependent manner. The shedding is believed to be controlled, at least in part, by enzyme action on the cell-associated capsular polysaccharide, heparosan. One candidate enzyme in such shedding is eliminase. The eliminase gene (elmA) was deleted from the genome of E. coli K5 and its effect on secreted and cell-associated heparosan was investigated. Deletion of the eliminase gene resulted in a significant reduction in heparosan shedding into the medium and heparosan content in the capsule of the cells, indicating its pivotal role in heparosan synthesis and shedding by E. coli K5.

Original languageEnglish
Pages (from-to)175-177
Number of pages3
JournalJournal of Biotechnology
Volume165
Issue number3-4
DOIs
StatePublished - Jun 2013
Externally publishedYes

Keywords

  • Bioengineered heparin
  • Capsular polysaccharide
  • Eliminase
  • Escherichia coli K5
  • Heparosan
  • Lyase

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