TY - JOUR
T1 - Effect of DNA repair protein Rad18 on viral infection
AU - Lloyd, Aliza G.
AU - Tateishi, Satoshi
AU - Bieniasz, Paul D.
AU - Muesing, Mark A.
AU - Yamaizumi, Masaru
AU - Mulder, Lubbertus C.F.
PY - 2006/5
Y1 - 2006/5
N2 - Host factors belonging to the DNA repair machineries are assumed to aid retroviruses in the obligatory step of integration. Here we describe the effect of DNA repair molecule Rad18, a component of the post-replication repair pathway, on viral infection. Contrary to our expectations, cells lacking Rad18 were consistently more permissive to viral transduction as compared to Rad18+/+ controls. Remarkably, such susceptibility was integration independent, since retroviruses devoid of integration activity also showed enhancement of the initial steps of infection. Moreover, the elevated sensitivity of the Rad18-/- cells was also observed with adenovirus. These data indicate that Rad18 suppresses viral infection in a non-specific fashion, probably by targeting incoming DNA. Furthermore, considering data published recently, it appears that the interactions between DNA repair components with incoming viruses, often result in inhibition of the infection rather than cooperation toward its establishment. Copyright:
AB - Host factors belonging to the DNA repair machineries are assumed to aid retroviruses in the obligatory step of integration. Here we describe the effect of DNA repair molecule Rad18, a component of the post-replication repair pathway, on viral infection. Contrary to our expectations, cells lacking Rad18 were consistently more permissive to viral transduction as compared to Rad18+/+ controls. Remarkably, such susceptibility was integration independent, since retroviruses devoid of integration activity also showed enhancement of the initial steps of infection. Moreover, the elevated sensitivity of the Rad18-/- cells was also observed with adenovirus. These data indicate that Rad18 suppresses viral infection in a non-specific fashion, probably by targeting incoming DNA. Furthermore, considering data published recently, it appears that the interactions between DNA repair components with incoming viruses, often result in inhibition of the infection rather than cooperation toward its establishment. Copyright:
UR - http://www.scopus.com/inward/record.url?scp=33646943507&partnerID=8YFLogxK
U2 - 10.1371/journal.ppat.0020040
DO - 10.1371/journal.ppat.0020040
M3 - Article
AN - SCOPUS:33646943507
SN - 1553-7366
VL - 2
SP - 368
EP - 373
JO - PLoS Pathogens
JF - PLoS Pathogens
IS - 5
ER -