TY - JOUR
T1 - Effect of different Epstein-Barr virus-determined antigens (EBNA, EA, and VCA) on the leukocyte migration of healthy donors and patients with infectious mononucleosis and certain immunodeficiencies
AU - Szigeti, R.
AU - Masucci, Maria G.
AU - Henle, W.
AU - Henle, Gertrude
AU - Purtilo, D.
AU - Klein, G.
N1 - Funding Information:
This study was supported (in part) by Contract NO1 CP 33316 from the Division of Cancer and Prevention, National Cancer Institute, the Swedish Cancer Society. King Gustaf V Jubilee
Funding Information:
and Contract NO1 CP 33272 with the Division of Cancer Detection and Prevention, Institute, U.S. Public Health Service. R. Szigeti was a recipient of a Guest Scholarship of the Swedish Institute, Stockholm, while on leave from the Second Department of Pediatrics, Semmelweis Medical University, Hungary. M. G. Masucci is a recipient of a fellowship from the Foundation Boncompagni-Ludovisi fiidd Bildt, Stockholm, Sweden. The authors wish to thank Barbro Ehlin, Mona Hedenskog, Sheila Kelly, and Marie Adams for skilled technical assistance, Dr. Ola Weiland (Roslagstull Hospital, Stockholm), Dr. Magnus Bjbrk-holm (Danderyd’s Hospital, Stockholm), Dr. Bo Johansson, Dr. HBkan Mellstedt, and Dr. Erik Svedmyr (Radiumhemmet, Karolinska Hospital, Stockholm) for the opportunity of testing their patients.
PY - 1982/1
Y1 - 1982/1
N2 - Leukocytes from healthy Epstein-Barr virus (EBV)-seropositive (SP) subjects show significant migration (LMI) with EBNA (EBV-determined nuclear antigen)-containing extracts derived from nonproducer, EBV-genome-carrying cells, or presented in the form of partially purified EBNA-preparation. Additional EBV antigens, EA and VCA, presented in the form of induced cell extracts do not add any measurable increase to their response. Leukocytes from acute infectious mononucleosis (IM) patients, on the other hand, do not respond to EBNA, but they show a good response to induced EA- or EA and VCA-containing cell extracts. This is in accordance with known differences in EBV-antibody pattern in healthy SPs as contrasted to acute IM patients. Leukocytes from chronic mononucleosis patients resemble those from acute IM patients in their preferential EA/VCA and deficient EBNA response in the LMI test. Cells from four X-linked lymphoproliferative disease (XLP) patients showed complete absence of reactivity to PHA and to EBV antigens in the LMI test, showing a general defect in cell-mediated immune responses. Leukocytes from a group of patients with lymphoproliferative malignancies in remission and elevated EBV-antibody titers showed a variety of responses, resembling normal SP donors, or IM patients, or showing a complete unresponsiveness in the LMI test.
AB - Leukocytes from healthy Epstein-Barr virus (EBV)-seropositive (SP) subjects show significant migration (LMI) with EBNA (EBV-determined nuclear antigen)-containing extracts derived from nonproducer, EBV-genome-carrying cells, or presented in the form of partially purified EBNA-preparation. Additional EBV antigens, EA and VCA, presented in the form of induced cell extracts do not add any measurable increase to their response. Leukocytes from acute infectious mononucleosis (IM) patients, on the other hand, do not respond to EBNA, but they show a good response to induced EA- or EA and VCA-containing cell extracts. This is in accordance with known differences in EBV-antibody pattern in healthy SPs as contrasted to acute IM patients. Leukocytes from chronic mononucleosis patients resemble those from acute IM patients in their preferential EA/VCA and deficient EBNA response in the LMI test. Cells from four X-linked lymphoproliferative disease (XLP) patients showed complete absence of reactivity to PHA and to EBV antigens in the LMI test, showing a general defect in cell-mediated immune responses. Leukocytes from a group of patients with lymphoproliferative malignancies in remission and elevated EBV-antibody titers showed a variety of responses, resembling normal SP donors, or IM patients, or showing a complete unresponsiveness in the LMI test.
UR - http://www.scopus.com/inward/record.url?scp=0020077157&partnerID=8YFLogxK
U2 - 10.1016/0090-1229(82)90029-0
DO - 10.1016/0090-1229(82)90029-0
M3 - Article
C2 - 6288294
AN - SCOPUS:0020077157
SN - 0090-1229
VL - 22
SP - 128
EP - 138
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
IS - 1
ER -