Effect of Chemical Modification on the Rate of Renaturation of Deoxyribonucleic Acid. Deaminated and Glyoxalated Deoxyribonucleic Acid

James R. Hutton; James G. Wetmur

doi:10.1021/bi00727a032

Effect of Chemical Modification on the Rate of Renaturation of Deoxyribonucleic Acid. Deaminated and Glyoxalated Deoxyribonucleic Acid

James R. Hutton, James G. Wetmur

Research output: Contribution to journal › Article › peer-review

93 Scopus citations

Abstract

Two model systems for DNA base mismatching have been studied. Deaminated DNA was investigated as a model for mismatches other than a purine–purine mismatch. Glyoxalated DNA was studied as a model for the purine–purine case. The melting temperature of glyoxalated DNA is found to be decreased by 1.1 ± 0.2° per mol of glyoxal bound per 100 base pairs. The renaturation rate of deaminated DNA is reduced by a factor of 2 when the melting temperature is lowered by 23°, corresponding to 33% mismatching. The renaturation rate of glyoxalated DNA is reduced by a factor of 2 when the melting temperature is lowered by 17°, corresponding to 16% mismatching. A theory is derived for modified DNA which accounts for the observed results.

Original language	English
Pages (from-to)	558-563
Number of pages	6
Journal	Biochemistry
Volume	12
Issue number	3
DOIs	https://doi.org/10.1021/bi00727a032
State	Published - 1 Jan 1973
Externally published	Yes

Access to Document

10.1021/bi00727a032

Cite this

@article{c4d4752002d447a9a0f84abb6540bbfd,

title = "Effect of Chemical Modification on the Rate of Renaturation of Deoxyribonucleic Acid. Deaminated and Glyoxalated Deoxyribonucleic Acid",

abstract = "Two model systems for DNA base mismatching have been studied. Deaminated DNA was investigated as a model for mismatches other than a purine–purine mismatch. Glyoxalated DNA was studied as a model for the purine–purine case. The melting temperature of glyoxalated DNA is found to be decreased by 1.1 ± 0.2° per mol of glyoxal bound per 100 base pairs. The renaturation rate of deaminated DNA is reduced by a factor of 2 when the melting temperature is lowered by 23°, corresponding to 33% mismatching. The renaturation rate of glyoxalated DNA is reduced by a factor of 2 when the melting temperature is lowered by 17°, corresponding to 16% mismatching. A theory is derived for modified DNA which accounts for the observed results.",

author = "Hutton, {James R.} and Wetmur, {James G.}",

year = "1973",

month = jan,

day = "1",

doi = "10.1021/bi00727a032",

language = "English",

volume = "12",

pages = "558--563",

journal = "Biochemistry",

issn = "0006-2960",

publisher = "American Chemical Society",

number = "3",

}

TY - JOUR

T1 - Effect of Chemical Modification on the Rate of Renaturation of Deoxyribonucleic Acid. Deaminated and Glyoxalated Deoxyribonucleic Acid

AU - Hutton, James R.

AU - Wetmur, James G.

PY - 1973/1/1

Y1 - 1973/1/1

N2 - Two model systems for DNA base mismatching have been studied. Deaminated DNA was investigated as a model for mismatches other than a purine–purine mismatch. Glyoxalated DNA was studied as a model for the purine–purine case. The melting temperature of glyoxalated DNA is found to be decreased by 1.1 ± 0.2° per mol of glyoxal bound per 100 base pairs. The renaturation rate of deaminated DNA is reduced by a factor of 2 when the melting temperature is lowered by 23°, corresponding to 33% mismatching. The renaturation rate of glyoxalated DNA is reduced by a factor of 2 when the melting temperature is lowered by 17°, corresponding to 16% mismatching. A theory is derived for modified DNA which accounts for the observed results.

AB - Two model systems for DNA base mismatching have been studied. Deaminated DNA was investigated as a model for mismatches other than a purine–purine mismatch. Glyoxalated DNA was studied as a model for the purine–purine case. The melting temperature of glyoxalated DNA is found to be decreased by 1.1 ± 0.2° per mol of glyoxal bound per 100 base pairs. The renaturation rate of deaminated DNA is reduced by a factor of 2 when the melting temperature is lowered by 23°, corresponding to 33% mismatching. The renaturation rate of glyoxalated DNA is reduced by a factor of 2 when the melting temperature is lowered by 17°, corresponding to 16% mismatching. A theory is derived for modified DNA which accounts for the observed results.

UR - http://www.scopus.com/inward/record.url?scp=0015939401&partnerID=8YFLogxK

U2 - 10.1021/bi00727a032

DO - 10.1021/bi00727a032

M3 - Article

C2 - 4683497

AN - SCOPUS:0015939401

SN - 0006-2960

VL - 12

SP - 558

EP - 563

JO - Biochemistry

JF - Biochemistry

IS - 3

ER -

Effect of Chemical Modification on the Rate of Renaturation of Deoxyribonucleic Acid. Deaminated and Glyoxalated Deoxyribonucleic Acid

Abstract

Access to Document

Fingerprint

Cite this