Effect of age on cytokine response in an experimental model of osteomyelitis

Felícito García-Alvarez, Marta Navarro-Zorraquino, Angel Castro, José María Grasa, Cristina Pastor, Marta Monzón, Ana Martínez, Ignacio García-Alvarez, Javier Castillo, Ricardo Lozano

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


To study the effect of age on cytokine response in an experimental model of osteomyelitis. Forty adult male Wistar rats received a stainless steel needle, intramedullarly in the left tibia. Young rats (3 months old) and old rats (22 months old) were allotted in: Group A: Sterile implant. Group B: Sterile implant + slime producing S. aureus. Rats were sacrificed 9 weeks after surgery. Determinations: Cytokines (ELISA) in blood and in tibia extract and the number of bacteria in tibia and implant. The Wilcoxon, Mann-Whitney Utests were used (P ≤ 0.01 significant). Infection was detected in every old rat receiving S. aureus, and in 7 of 10 young rats. In blood: prior to surgery, old rats presented higher IL-2 and lower IL-4 levels. Surgery alone did not induce significant changes in old rats; surgery + S. aureusinduced significant increases of IL-2 and IL-10 in young rats, and of IL-6 in old rats. Tibia analysis S. aureusgroup showed increased levels of: IL-10 in young rats, and IL-1β in old rats. In experimentally induced osteomyelitis, significant differences were observed in cytokine response with regard to age.

Original languageEnglish
Pages (from-to)649-658
Number of pages10
Issue number5
StatePublished - 2009
Externally publishedYes


  • Age
  • Cytokines
  • Implant
  • Osteomyelitis
  • Surgery


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