Effect of age on cytokine response in an experimental model of osteomyelitis

Felícito García-Alvarez; Marta Navarro-Zorraquino; Angel Castro; José María Grasa; Cristina Pastor; Marta Monzón; Ana Martínez; Ignacio García-Alvarez; Javier Castillo; Ricardo Lozano

doi:10.1007/s10522-008-9211-1

Effect of age on cytokine response in an experimental model of osteomyelitis

Felícito García-Alvarez, Marta Navarro-Zorraquino, Angel Castro, José María Grasa, Cristina Pastor, Marta Monzón, Ana Martínez, Ignacio García-Alvarez, Javier Castillo, Ricardo Lozano

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

To study the effect of age on cytokine response in an experimental model of osteomyelitis. Forty adult male Wistar rats received a stainless steel needle, intramedullarly in the left tibia. Young rats (3 months old) and old rats (22 months old) were allotted in: Group A: Sterile implant. Group B: Sterile implant + slime producing S. aureus. Rats were sacrificed 9 weeks after surgery. Determinations: Cytokines (ELISA) in blood and in tibia extract and the number of bacteria in tibia and implant. The Wilcoxon, Mann-Whitney Utests were used (P ≤ 0.01 significant). Infection was detected in every old rat receiving S. aureus, and in 7 of 10 young rats. In blood: prior to surgery, old rats presented higher IL-2 and lower IL-4 levels. Surgery alone did not induce significant changes in old rats; surgery + S. aureusinduced significant increases of IL-2 and IL-10 in young rats, and of IL-6 in old rats. Tibia analysis S. aureusgroup showed increased levels of: IL-10 in young rats, and IL-1β in old rats. In experimentally induced osteomyelitis, significant differences were observed in cytokine response with regard to age.

Original language	English
Pages (from-to)	649-658
Number of pages	10
Journal	Biogerontology
Volume	10
Issue number	5
DOIs	https://doi.org/10.1007/s10522-008-9211-1
State	Published - 2009
Externally published	Yes

Keywords

Age
Cytokines
Implant
Osteomyelitis
Surgery

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10.1007/s10522-008-9211-1

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@article{54d3308b47ee43e2a27ea8a96ec83502,

title = "Effect of age on cytokine response in an experimental model of osteomyelitis",

abstract = "To study the effect of age on cytokine response in an experimental model of osteomyelitis. Forty adult male Wistar rats received a stainless steel needle, intramedullarly in the left tibia. Young rats (3 months old) and old rats (22 months old) were allotted in: Group A: Sterile implant. Group B: Sterile implant + slime producing S. aureus. Rats were sacrificed 9 weeks after surgery. Determinations: Cytokines (ELISA) in blood and in tibia extract and the number of bacteria in tibia and implant. The Wilcoxon, Mann-Whitney Utests were used (P ≤ 0.01 significant). Infection was detected in every old rat receiving S. aureus, and in 7 of 10 young rats. In blood: prior to surgery, old rats presented higher IL-2 and lower IL-4 levels. Surgery alone did not induce significant changes in old rats; surgery + S. aureusinduced significant increases of IL-2 and IL-10 in young rats, and of IL-6 in old rats. Tibia analysis S. aureusgroup showed increased levels of: IL-10 in young rats, and IL-1β in old rats. In experimentally induced osteomyelitis, significant differences were observed in cytokine response with regard to age.",

keywords = "Age, Cytokines, Implant, Osteomyelitis, Surgery",

author = "Fel{\'i}cito Garc{\'i}a-Alvarez and Marta Navarro-Zorraquino and Angel Castro and Grasa, {Jos{\'e} Mar{\'i}a} and Cristina Pastor and Marta Monz{\'o}n and Ana Mart{\'i}nez and Ignacio Garc{\'i}a-Alvarez and Javier Castillo and Ricardo Lozano",

note = "Funding Information: Acknowledgments This work was supported by Grant SAF2004-03986 from the Ministerio de Educaci{\'o}n y Ciencia de Espa{\~n}a-European Council.",

year = "2009",

doi = "10.1007/s10522-008-9211-1",

language = "English",

volume = "10",

pages = "649--658",

journal = "Biogerontology",

issn = "1389-5729",

publisher = "Springer Netherlands",

number = "5",

}

TY - JOUR

T1 - Effect of age on cytokine response in an experimental model of osteomyelitis

AU - García-Alvarez, Felícito

AU - Navarro-Zorraquino, Marta

AU - Castro, Angel

AU - Grasa, José María

AU - Pastor, Cristina

AU - Monzón, Marta

AU - Martínez, Ana

AU - García-Alvarez, Ignacio

AU - Castillo, Javier

AU - Lozano, Ricardo

N1 - Funding Information: Acknowledgments This work was supported by Grant SAF2004-03986 from the Ministerio de Educación y Ciencia de España-European Council.

PY - 2009

Y1 - 2009

N2 - To study the effect of age on cytokine response in an experimental model of osteomyelitis. Forty adult male Wistar rats received a stainless steel needle, intramedullarly in the left tibia. Young rats (3 months old) and old rats (22 months old) were allotted in: Group A: Sterile implant. Group B: Sterile implant + slime producing S. aureus. Rats were sacrificed 9 weeks after surgery. Determinations: Cytokines (ELISA) in blood and in tibia extract and the number of bacteria in tibia and implant. The Wilcoxon, Mann-Whitney Utests were used (P ≤ 0.01 significant). Infection was detected in every old rat receiving S. aureus, and in 7 of 10 young rats. In blood: prior to surgery, old rats presented higher IL-2 and lower IL-4 levels. Surgery alone did not induce significant changes in old rats; surgery + S. aureusinduced significant increases of IL-2 and IL-10 in young rats, and of IL-6 in old rats. Tibia analysis S. aureusgroup showed increased levels of: IL-10 in young rats, and IL-1β in old rats. In experimentally induced osteomyelitis, significant differences were observed in cytokine response with regard to age.

AB - To study the effect of age on cytokine response in an experimental model of osteomyelitis. Forty adult male Wistar rats received a stainless steel needle, intramedullarly in the left tibia. Young rats (3 months old) and old rats (22 months old) were allotted in: Group A: Sterile implant. Group B: Sterile implant + slime producing S. aureus. Rats were sacrificed 9 weeks after surgery. Determinations: Cytokines (ELISA) in blood and in tibia extract and the number of bacteria in tibia and implant. The Wilcoxon, Mann-Whitney Utests were used (P ≤ 0.01 significant). Infection was detected in every old rat receiving S. aureus, and in 7 of 10 young rats. In blood: prior to surgery, old rats presented higher IL-2 and lower IL-4 levels. Surgery alone did not induce significant changes in old rats; surgery + S. aureusinduced significant increases of IL-2 and IL-10 in young rats, and of IL-6 in old rats. Tibia analysis S. aureusgroup showed increased levels of: IL-10 in young rats, and IL-1β in old rats. In experimentally induced osteomyelitis, significant differences were observed in cytokine response with regard to age.

KW - Age

KW - Cytokines

KW - Implant

KW - Osteomyelitis

KW - Surgery

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U2 - 10.1007/s10522-008-9211-1

DO - 10.1007/s10522-008-9211-1

M3 - Article

C2 - 19123052

AN - SCOPUS:69249214184

SN - 1389-5729

VL - 10

SP - 649

EP - 658

JO - Biogerontology

JF - Biogerontology

IS - 5

ER -

Effect of age on cytokine response in an experimental model of osteomyelitis

Abstract

Keywords

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