Early expression of mannose-binding lectin 2 during Aspergillus fumigatus infection in human corneal epithelial cells

Cheng Ye Che; Jing Fen Zhang; Ji Eun Lee; Jing Lin; Li Ting Hu; Nan Jiang; Qian Wang; Qiang Xu; Gui Qiu Zhao

doi:10.3980/j.issn.2222-3959.2015.01.06

Early expression of mannose-binding lectin 2 during Aspergillus fumigatus infection in human corneal epithelial cells

Cheng Ye Che, Jing Fen Zhang, Ji Eun Lee, Jing Lin, Li Ting Hu, Nan Jiang, Qian Wang, Qiang Xu, Gui Qiu Zhao

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

AIM To evaluate the early expression of mannose-binding lectin 2 (MBL2) in human corneal epithelial cells (HCECs) infected by Aspergillus fumigatus (AF). METHODS HCECs cultured in vitro with AF antigens and sampled at 0, 0.5, 1, 2, 4, 6 and 8h. The expression of MBL2 mRNA was evaluated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The expression of MBL2 protein in supernatant fluid was shown by enzyme linked immunosorbent assay (ELISA). MBL2 protein in HCECs was detected by immunocytochemistry at 0 and 24h. RESULTS MBL2 mRNA and protein are expressed in normal HCECs. The expression of MBL2 mRNA and protein in supernatant fluid begin to increase after being stimulated with AF antigens. The most significantly peak of MBL2 mRNA is in 2h. The protein of MBL2 in supernatant fluid decrease gradually after 0.5h. The protein in HCECs expression increase after stimulation of 24h. CONCLUSION MBL2 receptor expressed in normal HCECs in vitro. The stimulation by AF antigens can increase the early expression of it.

Original language	English
Pages (from-to)	35-38
Number of pages	4
Journal	International Journal of Ophthalmology
Volume	8
Issue number	1
DOIs	https://doi.org/10.3980/j.issn.2222-3959.2015.01.06
State	Published - 18 Feb 2015
Externally published	Yes

Keywords

Aspergillus fumigatus
Human corneal epithelial cells
Mannose-binding lectin

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10.3980/j.issn.2222-3959.2015.01.06

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@article{ac1138615f8047079d068a7f7414e9f2,

title = "Early expression of mannose-binding lectin 2 during Aspergillus fumigatus infection in human corneal epithelial cells",

abstract = "AIM To evaluate the early expression of mannose-binding lectin 2 (MBL2) in human corneal epithelial cells (HCECs) infected by Aspergillus fumigatus (AF). METHODS HCECs cultured in vitro with AF antigens and sampled at 0, 0.5, 1, 2, 4, 6 and 8h. The expression of MBL2 mRNA was evaluated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The expression of MBL2 protein in supernatant fluid was shown by enzyme linked immunosorbent assay (ELISA). MBL2 protein in HCECs was detected by immunocytochemistry at 0 and 24h. RESULTS MBL2 mRNA and protein are expressed in normal HCECs. The expression of MBL2 mRNA and protein in supernatant fluid begin to increase after being stimulated with AF antigens. The most significantly peak of MBL2 mRNA is in 2h. The protein of MBL2 in supernatant fluid decrease gradually after 0.5h. The protein in HCECs expression increase after stimulation of 24h. CONCLUSION MBL2 receptor expressed in normal HCECs in vitro. The stimulation by AF antigens can increase the early expression of it.",

keywords = "Aspergillus fumigatus, Human corneal epithelial cells, Mannose-binding lectin",

author = "Che, {Cheng Ye} and Zhang, {Jing Fen} and Lee, {Ji Eun} and Jing Lin and Hu, {Li Ting} and Nan Jiang and Qian Wang and Qiang Xu and Zhao, {Gui Qiu}",

year = "2015",

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doi = "10.3980/j.issn.2222-3959.2015.01.06",

language = "English",

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pages = "35--38",

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TY - JOUR

T1 - Early expression of mannose-binding lectin 2 during Aspergillus fumigatus infection in human corneal epithelial cells

AU - Che, Cheng Ye

AU - Zhang, Jing Fen

AU - Lee, Ji Eun

AU - Lin, Jing

AU - Hu, Li Ting

AU - Jiang, Nan

AU - Wang, Qian

AU - Xu, Qiang

AU - Zhao, Gui Qiu

PY - 2015/2/18

Y1 - 2015/2/18

N2 - AIM To evaluate the early expression of mannose-binding lectin 2 (MBL2) in human corneal epithelial cells (HCECs) infected by Aspergillus fumigatus (AF). METHODS HCECs cultured in vitro with AF antigens and sampled at 0, 0.5, 1, 2, 4, 6 and 8h. The expression of MBL2 mRNA was evaluated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The expression of MBL2 protein in supernatant fluid was shown by enzyme linked immunosorbent assay (ELISA). MBL2 protein in HCECs was detected by immunocytochemistry at 0 and 24h. RESULTS MBL2 mRNA and protein are expressed in normal HCECs. The expression of MBL2 mRNA and protein in supernatant fluid begin to increase after being stimulated with AF antigens. The most significantly peak of MBL2 mRNA is in 2h. The protein of MBL2 in supernatant fluid decrease gradually after 0.5h. The protein in HCECs expression increase after stimulation of 24h. CONCLUSION MBL2 receptor expressed in normal HCECs in vitro. The stimulation by AF antigens can increase the early expression of it.

AB - AIM To evaluate the early expression of mannose-binding lectin 2 (MBL2) in human corneal epithelial cells (HCECs) infected by Aspergillus fumigatus (AF). METHODS HCECs cultured in vitro with AF antigens and sampled at 0, 0.5, 1, 2, 4, 6 and 8h. The expression of MBL2 mRNA was evaluated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The expression of MBL2 protein in supernatant fluid was shown by enzyme linked immunosorbent assay (ELISA). MBL2 protein in HCECs was detected by immunocytochemistry at 0 and 24h. RESULTS MBL2 mRNA and protein are expressed in normal HCECs. The expression of MBL2 mRNA and protein in supernatant fluid begin to increase after being stimulated with AF antigens. The most significantly peak of MBL2 mRNA is in 2h. The protein of MBL2 in supernatant fluid decrease gradually after 0.5h. The protein in HCECs expression increase after stimulation of 24h. CONCLUSION MBL2 receptor expressed in normal HCECs in vitro. The stimulation by AF antigens can increase the early expression of it.

KW - Aspergillus fumigatus

KW - Human corneal epithelial cells

KW - Mannose-binding lectin

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U2 - 10.3980/j.issn.2222-3959.2015.01.06

DO - 10.3980/j.issn.2222-3959.2015.01.06

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Early expression of mannose-binding lectin 2 during Aspergillus fumigatus infection in human corneal epithelial cells

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