TY - JOUR
T1 - Early DNA-binding polypeptides of Epstein-Barr virus
AU - Roubal, Jaroslav
AU - Kallin, Bengt
AU - Luka, Janos
AU - Klein, George
N1 - Funding Information:
This research was supported (in part) by Contract No. NO1 CP 33316 from the Division of Cancer Cause and Prevention, National Cancer Institute, and the Swedish Cancer Society. The work reported in this paper was undertaken during the tenure of a Research Training Fellowship awarded to J.R. by the International Agency for Research on Cancer.
PY - 1981/8
Y1 - 1981/8
N2 - The kinetics of Epstein-Barr virus (EBV)-specific protein synthesis has been studied after induction of P3HR-1 cells with n-butyrate. The majority of the EBV polypeptides became detectable on SDS-polyacrylamide gel electrophoresis between 15 and 18 hr after addition of the drug. Overall viral protein synthesis was at maximum after about 24 hr. Some polypeptides appeared significantly earlier (152K, 55K, 51K) while others were synthesized during a limited period of the cycle only (65K). At least four of the polypeptides detected (152K, 134K, 55K, 51K) bound to DNA cellulose columns. The EBV specificity of the polypeptides was confirmed by immunoprecipitation and by two-dimensional gel analysis. The 152K polypeptide had a pI around 8 whereas the other polypeptides varied between basic and acidic pI. The DNA binding proteins isolated by DNA cellulose chromatography represent a subclass of the virus-specific early proteins that can be immunoprecipitated from the total cell extract.
AB - The kinetics of Epstein-Barr virus (EBV)-specific protein synthesis has been studied after induction of P3HR-1 cells with n-butyrate. The majority of the EBV polypeptides became detectable on SDS-polyacrylamide gel electrophoresis between 15 and 18 hr after addition of the drug. Overall viral protein synthesis was at maximum after about 24 hr. Some polypeptides appeared significantly earlier (152K, 55K, 51K) while others were synthesized during a limited period of the cycle only (65K). At least four of the polypeptides detected (152K, 134K, 55K, 51K) bound to DNA cellulose columns. The EBV specificity of the polypeptides was confirmed by immunoprecipitation and by two-dimensional gel analysis. The 152K polypeptide had a pI around 8 whereas the other polypeptides varied between basic and acidic pI. The DNA binding proteins isolated by DNA cellulose chromatography represent a subclass of the virus-specific early proteins that can be immunoprecipitated from the total cell extract.
UR - https://www.scopus.com/pages/publications/0019600764
U2 - 10.1016/0042-6822(81)90155-0
DO - 10.1016/0042-6822(81)90155-0
M3 - Article
C2 - 6267784
AN - SCOPUS:0019600764
SN - 0042-6822
VL - 113
SP - 285
EP - 292
JO - Virology
JF - Virology
IS - 1
ER -