TY - JOUR
T1 - Early DNA-binding polypeptides of Epstein-Barr virus
AU - Roubal, J.
AU - Kallin, B.
AU - Luka, J.
AU - Klein, G.
N1 - Funding Information:
This research was supported (in part) by Contract No. NO1 CP 33316 from the Division of Cancer Cause and Prevention, National Cancer Institute, and the Swedish Cancer Society. The work reported in this paper was undertaken during the tenure of a Research Training Fellowship awarded to J.R. by the International Agency for Research on Cancer.
PY - 1981
Y1 - 1981
N2 - The kinetics of Epstein-Barr virus (EBV)-specific protein synthesis has been studied after incubation of P3HR-1 cells with n-butyrate. The majority of the EBV polypeptides became detectable on SDS-polyacrylamide gel electrophoresis between 15 and 18 hr after addition of the drug. Overall viral protein synthesis was at maximum after about 24 hr. Some polypeptides appeared significantly earlier (152K, 55K, 51K) while others were synthesized during a limited period of the cycle only (65K). At least four of the polypeptides detected (152K, 134K, 55K, 51K) bound to DNA cellulose columns. The EBV specificity of the polypeptides was confirmed by immunoprecipitation and by two-dimensional gel analysis. The 152K polypeptide had a pI around 8 whereas the other polypeptides varied between basic and acidic pI. The DNA binding proteins isolated by DNA cellulose chromatography represent a subclass of the virus-specific early proteins that can be immunoprecipitated from the total cell extract.
AB - The kinetics of Epstein-Barr virus (EBV)-specific protein synthesis has been studied after incubation of P3HR-1 cells with n-butyrate. The majority of the EBV polypeptides became detectable on SDS-polyacrylamide gel electrophoresis between 15 and 18 hr after addition of the drug. Overall viral protein synthesis was at maximum after about 24 hr. Some polypeptides appeared significantly earlier (152K, 55K, 51K) while others were synthesized during a limited period of the cycle only (65K). At least four of the polypeptides detected (152K, 134K, 55K, 51K) bound to DNA cellulose columns. The EBV specificity of the polypeptides was confirmed by immunoprecipitation and by two-dimensional gel analysis. The 152K polypeptide had a pI around 8 whereas the other polypeptides varied between basic and acidic pI. The DNA binding proteins isolated by DNA cellulose chromatography represent a subclass of the virus-specific early proteins that can be immunoprecipitated from the total cell extract.
UR - http://www.scopus.com/inward/record.url?scp=0019469061&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0019469061
SN - 0166-4328
VL - 3
SP - 285
EP - 292
JO - Behavioural Brain Research
JF - Behavioural Brain Research
IS - 2
ER -