Dysbiosis of Gut Microbiome Is Associated with Rupture of Cerebral Aneurysms

Shuhei Kawabata; Masatoshi Takagaki; Hajime Nakamura; Hiroya Oki; Daisuke Motooka; Shota Nakamura; Takeo Nishida; Eisaku Terada; Nobuyuki Izutsu; Tomofumi Takenaka; Yuichi Matsui; Shuhei Yamada; Katsunori Asai; Akihiro Tateishi; Toru Umehara; Yoshihiro Yano; Yohei Bamba; Katsumi Matsumoto; Toshihiro Kishikawa; Yukinori Okada; Tetsuya Iida; Haruhiko Kishima

doi:10.1161/STROKEAHA.121.034792

Dysbiosis of Gut Microbiome Is Associated with Rupture of Cerebral Aneurysms

Shuhei Kawabata
, Masatoshi Takagaki
, Hajime Nakamura
, Hiroya Oki
, Daisuke Motooka
, Shota Nakamura
, Takeo Nishida
, Eisaku Terada
, Nobuyuki Izutsu
, Tomofumi Takenaka
, Yuichi Matsui
, Shuhei Yamada
, Katsunori Asai
, Akihiro Tateishi
, Toru Umehara
, Yoshihiro Yano
, Yohei Bamba
, Katsumi Matsumoto
, Toshihiro Kishikawa
, Yukinori Okada

Tetsuya Iida, Haruhiko Kishima

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Background and Purpose: Environmental factors are important with respect to the rupture of cerebral aneurysms. However, the relationship between the gut microbiome, an environmental factor, and aneurysm rupture is unclear. Therefore, we compared the gut microbiome in patients with unruptured intracranial aneurysms (UIAs) and ruptured aneurysms (RAs) to identify the specific bacteria causing the rupture of cerebral aneurysms. Methods: A multicenter, prospective case-control study was conducted over one year from 2019 to 2020. The fecal samples of patients with stable UIAs and RAs immediately after onset were collected. Their gut microbiomes were analyzed using 16S rRNA sequencing. Subsequently, a phylogenetic tree was constructed, and polymerase chain reaction was performed to identify the specific species. Results: A total of 28 RAs and 33 UIAs were included in this study. There was no difference in patient characteristics between RAs and UIAs: age, sex, hypertension, dyslipidemia, diabetes status, body mass index, and smoking. No difference was observed in alpha diversity; however, beta diversity was significantly different in the unweighted UniFrac distances. At the phylum level, the relative abundance of Campylobacter in the RA group was larger than that in the UIA group. Furthermore, the gut microbiome in the RA and UIA groups exhibited significantly different taxonomies. However, Campylobacter was focused on because it is widely known as pathogenic among these bacteria. Then, a phylogenetic tree of operational taxonomic units related to Campylobacter was constructed and 4 species were identified. Polymerase chain reaction for these species identified that the abundance of the genus Campylobacter and Campylobacter ureolyticus was significantly higher in the RA group. Conclusions: The gut microbiome profile of patients with stable UIAs and RAs were significantly different. The genus Campylobacter and Campylobacter ureolyticus may be associated with the rupture of cerebral aneurysms.

Original language	English
Pages (from-to)	895-903
Number of pages	9
Journal	Stroke
Volume	29
Issue number	2
DOIs	https://doi.org/10.1161/STROKEAHA.121.034792
State	Published - 1 Mar 2022
Externally published	Yes

Keywords

aneurysm
gut microbiome
inflammation
rupture

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10.1161/STROKEAHA.121.034792

Cite this

Kawabata, S., Takagaki, M., Nakamura, H., Oki, H., Motooka, D., Nakamura, S., Nishida, T., Terada, E., Izutsu, N., Takenaka, T., Matsui, Y., Yamada, S., Asai, K., Tateishi, A., Umehara, T., Yano, Y., Bamba, Y., Matsumoto, K., Kishikawa, T., ... Kishima, H. (2022). Dysbiosis of Gut Microbiome Is Associated with Rupture of Cerebral Aneurysms. Stroke, 29(2), 895-903. https://doi.org/10.1161/STROKEAHA.121.034792

@article{3c547be806c84bcf867b8695c6cc7618,

title = "Dysbiosis of Gut Microbiome Is Associated with Rupture of Cerebral Aneurysms",

abstract = "Background and Purpose: Environmental factors are important with respect to the rupture of cerebral aneurysms. However, the relationship between the gut microbiome, an environmental factor, and aneurysm rupture is unclear. Therefore, we compared the gut microbiome in patients with unruptured intracranial aneurysms (UIAs) and ruptured aneurysms (RAs) to identify the specific bacteria causing the rupture of cerebral aneurysms. Methods: A multicenter, prospective case-control study was conducted over one year from 2019 to 2020. The fecal samples of patients with stable UIAs and RAs immediately after onset were collected. Their gut microbiomes were analyzed using 16S rRNA sequencing. Subsequently, a phylogenetic tree was constructed, and polymerase chain reaction was performed to identify the specific species. Results: A total of 28 RAs and 33 UIAs were included in this study. There was no difference in patient characteristics between RAs and UIAs: age, sex, hypertension, dyslipidemia, diabetes status, body mass index, and smoking. No difference was observed in alpha diversity; however, beta diversity was significantly different in the unweighted UniFrac distances. At the phylum level, the relative abundance of Campylobacter in the RA group was larger than that in the UIA group. Furthermore, the gut microbiome in the RA and UIA groups exhibited significantly different taxonomies. However, Campylobacter was focused on because it is widely known as pathogenic among these bacteria. Then, a phylogenetic tree of operational taxonomic units related to Campylobacter was constructed and 4 species were identified. Polymerase chain reaction for these species identified that the abundance of the genus Campylobacter and Campylobacter ureolyticus was significantly higher in the RA group. Conclusions: The gut microbiome profile of patients with stable UIAs and RAs were significantly different. The genus Campylobacter and Campylobacter ureolyticus may be associated with the rupture of cerebral aneurysms.",

keywords = "aneurysm, gut microbiome, inflammation, rupture",

author = "Shuhei Kawabata and Masatoshi Takagaki and Hajime Nakamura and Hiroya Oki and Daisuke Motooka and Shota Nakamura and Takeo Nishida and Eisaku Terada and Nobuyuki Izutsu and Tomofumi Takenaka and Yuichi Matsui and Shuhei Yamada and Katsunori Asai and Akihiro Tateishi and Toru Umehara and Yoshihiro Yano and Yohei Bamba and Katsumi Matsumoto and Toshihiro Kishikawa and Yukinori Okada and Tetsuya Iida and Haruhiko Kishima",

year = "2022",

month = mar,

day = "1",

doi = "10.1161/STROKEAHA.121.034792",

language = "English",

volume = "29",

pages = "895--903",

journal = "Stroke",

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publisher = "Wolters Kluwer Health",

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Kawabata, S, Takagaki, M, Nakamura, H, Oki, H, Motooka, D, Nakamura, S, Nishida, T, Terada, E, Izutsu, N, Takenaka, T, Matsui, Y, Yamada, S, Asai, K, Tateishi, A, Umehara, T, Yano, Y, Bamba, Y, Matsumoto, K, Kishikawa, T, Okada, Y, Iida, T & Kishima, H 2022, 'Dysbiosis of Gut Microbiome Is Associated with Rupture of Cerebral Aneurysms', Stroke, vol. 29, no. 2, pp. 895-903. https://doi.org/10.1161/STROKEAHA.121.034792

TY - JOUR

T1 - Dysbiosis of Gut Microbiome Is Associated with Rupture of Cerebral Aneurysms

AU - Kawabata, Shuhei

AU - Takagaki, Masatoshi

AU - Nakamura, Hajime

AU - Oki, Hiroya

AU - Motooka, Daisuke

AU - Nakamura, Shota

AU - Nishida, Takeo

AU - Terada, Eisaku

AU - Izutsu, Nobuyuki

AU - Takenaka, Tomofumi

AU - Matsui, Yuichi

AU - Yamada, Shuhei

AU - Asai, Katsunori

AU - Tateishi, Akihiro

AU - Umehara, Toru

AU - Yano, Yoshihiro

AU - Bamba, Yohei

AU - Matsumoto, Katsumi

AU - Kishikawa, Toshihiro

AU - Okada, Yukinori

AU - Iida, Tetsuya

AU - Kishima, Haruhiko

PY - 2022/3/1

Y1 - 2022/3/1

N2 - Background and Purpose: Environmental factors are important with respect to the rupture of cerebral aneurysms. However, the relationship between the gut microbiome, an environmental factor, and aneurysm rupture is unclear. Therefore, we compared the gut microbiome in patients with unruptured intracranial aneurysms (UIAs) and ruptured aneurysms (RAs) to identify the specific bacteria causing the rupture of cerebral aneurysms. Methods: A multicenter, prospective case-control study was conducted over one year from 2019 to 2020. The fecal samples of patients with stable UIAs and RAs immediately after onset were collected. Their gut microbiomes were analyzed using 16S rRNA sequencing. Subsequently, a phylogenetic tree was constructed, and polymerase chain reaction was performed to identify the specific species. Results: A total of 28 RAs and 33 UIAs were included in this study. There was no difference in patient characteristics between RAs and UIAs: age, sex, hypertension, dyslipidemia, diabetes status, body mass index, and smoking. No difference was observed in alpha diversity; however, beta diversity was significantly different in the unweighted UniFrac distances. At the phylum level, the relative abundance of Campylobacter in the RA group was larger than that in the UIA group. Furthermore, the gut microbiome in the RA and UIA groups exhibited significantly different taxonomies. However, Campylobacter was focused on because it is widely known as pathogenic among these bacteria. Then, a phylogenetic tree of operational taxonomic units related to Campylobacter was constructed and 4 species were identified. Polymerase chain reaction for these species identified that the abundance of the genus Campylobacter and Campylobacter ureolyticus was significantly higher in the RA group. Conclusions: The gut microbiome profile of patients with stable UIAs and RAs were significantly different. The genus Campylobacter and Campylobacter ureolyticus may be associated with the rupture of cerebral aneurysms.

AB - Background and Purpose: Environmental factors are important with respect to the rupture of cerebral aneurysms. However, the relationship between the gut microbiome, an environmental factor, and aneurysm rupture is unclear. Therefore, we compared the gut microbiome in patients with unruptured intracranial aneurysms (UIAs) and ruptured aneurysms (RAs) to identify the specific bacteria causing the rupture of cerebral aneurysms. Methods: A multicenter, prospective case-control study was conducted over one year from 2019 to 2020. The fecal samples of patients with stable UIAs and RAs immediately after onset were collected. Their gut microbiomes were analyzed using 16S rRNA sequencing. Subsequently, a phylogenetic tree was constructed, and polymerase chain reaction was performed to identify the specific species. Results: A total of 28 RAs and 33 UIAs were included in this study. There was no difference in patient characteristics between RAs and UIAs: age, sex, hypertension, dyslipidemia, diabetes status, body mass index, and smoking. No difference was observed in alpha diversity; however, beta diversity was significantly different in the unweighted UniFrac distances. At the phylum level, the relative abundance of Campylobacter in the RA group was larger than that in the UIA group. Furthermore, the gut microbiome in the RA and UIA groups exhibited significantly different taxonomies. However, Campylobacter was focused on because it is widely known as pathogenic among these bacteria. Then, a phylogenetic tree of operational taxonomic units related to Campylobacter was constructed and 4 species were identified. Polymerase chain reaction for these species identified that the abundance of the genus Campylobacter and Campylobacter ureolyticus was significantly higher in the RA group. Conclusions: The gut microbiome profile of patients with stable UIAs and RAs were significantly different. The genus Campylobacter and Campylobacter ureolyticus may be associated with the rupture of cerebral aneurysms.

KW - aneurysm

KW - gut microbiome

KW - inflammation

KW - rupture

UR - https://www.scopus.com/pages/publications/85122453413

U2 - 10.1161/STROKEAHA.121.034792

DO - 10.1161/STROKEAHA.121.034792

M3 - Article

C2 - 34727738

AN - SCOPUS:85122453413

SN - 0039-2499

VL - 29

SP - 895

EP - 903

JO - Stroke

JF - Stroke

IS - 2

ER -

Dysbiosis of Gut Microbiome Is Associated with Rupture of Cerebral Aneurysms

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Keywords

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