@inproceedings{543362c1bfbc474a917e0eafca8e90b1,
title = "Dynamic AFM elastography reveals phase dependent mechanical heterogeneity of beating cardiac myocytes",
abstract = "We developed a novel atomic force microscope (AFM) indentation technique for mapping spatiotemporal stiffness of spontaneously beating neonatal rat cardiac myocytes. Cells were indented at a rate close but unequal to their contractile frequency. Resultant apparent elastic modulus cycled at a predictable envelope frequency between a systolic value of 26.2 ± 5.1 kPa and a diastolic value of 7.8 ± 4.1 kPa. In cells probed along their axis, spatial heterogeneity of systolic stiffness correlated with the sarcomeric structure of underlying myofibrils. Treatment with blebbistatin eliminated contractile activity and resulted in a uniform modulus of 6.5 ± 4.8 kPa. The technique provides a unique means of probing the mechanical effects of disease processes and pharmacological treatments on beating cardiomyocytes at the subcellular level, providing new insights relating myocardial structure and function.",
author = "Azeloglu, {Evren U.} and Costa, {Kevin D.}",
year = "2009",
doi = "10.1109/IEMBS.2009.5335316",
language = "English",
isbn = "9781424432967",
series = "Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009",
publisher = "IEEE Computer Society",
pages = "7180--7183",
booktitle = "Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society",
address = "United States",
note = "31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009 ; Conference date: 02-09-2009 Through 06-09-2009",
}