Dramatic stabilization of proteins adsorbed onto C-60 fullerenes

Prashanth Asuri, Sandeep S. Karajanagi, Ravi S. Kane, Jonathan S. Dordick

Research output: Contribution to conferencePaperpeer-review

Abstract

Designing highly active and stable protein preparations is critical for a variety of applications including biosensors, biomedical devices, functional materials, and surface coatings. We demonstrate the ability to enhance protein function and stability by controlling its nanoscale environment. Activities of fullerene-enzyme conjugates were significantly higher than the native enzyme and enzymes adsorbed on other carbonaceous supports, when subjected to elevated temperatures and organic solvents. The degree of stabilization strongly correlates with the unique surface curvature of the nanoscale materials, which appears to reduce undesirable protein-protein interactions. The enhanced stability was exploited in the preparation of highly active polymer- nanocomposite films that were highly stable under harsh environments. The stable fullerene-protein conjugates represent a new generation of highly selective, active, and stable catalytic materials with a major impact on applications ranging from protein chips, vaccines, and biofuel cells to drug delivery.

Original languageEnglish
Pages8390
Number of pages1
StatePublished - 2005
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States
Duration: 30 Oct 20054 Nov 2005

Conference

Conference05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
Country/TerritoryUnited States
CityCincinnati, OH
Period30/10/054/11/05

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