TY - JOUR
T1 - Dopamine- and cAMP-regulated phosphoprotein (DARPP-32) and dopamine DA1 agonist-sensitive Na+,K+-ATPase in renal tubule cells
AU - Meister, B.
AU - Fryckstedt, J.
AU - Schalling, M.
AU - Cortes, R.
AU - Hokfelt, T.
AU - Aperia, A.
AU - Hemmings, H. C.
AU - Nairn, A. C.
AU - Ehrlich, M.
AU - Greengard, P.
PY - 1989
Y1 - 1989
N2 - The cellular localization of DARPP-32, a dopamine- and cAMP-regulated phosphoprotein M(r) 32,000 that appears to mediate certain actions of dopamine in the mammalian brain by acting as an inhibitor of protein phosphatase 1, was studied in the kidney of several species. DARPP-32 mRNA and DARPP-32-like immunoreactivity were found in the cytoplasm of cells in the thick ascending limb of the loop of Henle. The specific dopamine DA1 agonist SKF 82526 caused a dose-dependent inhibition of Na+,K+-ATPase activity, which could be blocked by SCH 23390, a specific DA1 antagonist, and by PKI-(5-24) amide, a specific inhibitor of cAMP-dependent protein kinase. The results indicate that DA1 dopamine receptors and DARPP-32, an intracellular third messenger for dopamine, are part of the signal-transduction process for dopamine acting on renal tubule cells.
AB - The cellular localization of DARPP-32, a dopamine- and cAMP-regulated phosphoprotein M(r) 32,000 that appears to mediate certain actions of dopamine in the mammalian brain by acting as an inhibitor of protein phosphatase 1, was studied in the kidney of several species. DARPP-32 mRNA and DARPP-32-like immunoreactivity were found in the cytoplasm of cells in the thick ascending limb of the loop of Henle. The specific dopamine DA1 agonist SKF 82526 caused a dose-dependent inhibition of Na+,K+-ATPase activity, which could be blocked by SCH 23390, a specific DA1 antagonist, and by PKI-(5-24) amide, a specific inhibitor of cAMP-dependent protein kinase. The results indicate that DA1 dopamine receptors and DARPP-32, an intracellular third messenger for dopamine, are part of the signal-transduction process for dopamine acting on renal tubule cells.
UR - http://www.scopus.com/inward/record.url?scp=0344791242&partnerID=8YFLogxK
U2 - 10.1073/pnas.86.20.8068
DO - 10.1073/pnas.86.20.8068
M3 - Article
C2 - 2573060
AN - SCOPUS:0344791242
SN - 0027-8424
VL - 86
SP - 8068
EP - 8072
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 20
ER -