Dopamine- and cAMP-regulated phosphoprotein (DARPP-32) and dopamine DA1 agonist-sensitive Na+,K+-ATPase in renal tubule cells

B. Meister, J. Fryckstedt, M. Schalling, R. Cortes, T. Hokfelt, A. Aperia, H. C. Hemmings, A. C. Nairn, M. Ehrlich, P. Greengard

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106 Scopus citations

Abstract

The cellular localization of DARPP-32, a dopamine- and cAMP-regulated phosphoprotein M(r) 32,000 that appears to mediate certain actions of dopamine in the mammalian brain by acting as an inhibitor of protein phosphatase 1, was studied in the kidney of several species. DARPP-32 mRNA and DARPP-32-like immunoreactivity were found in the cytoplasm of cells in the thick ascending limb of the loop of Henle. The specific dopamine DA1 agonist SKF 82526 caused a dose-dependent inhibition of Na+,K+-ATPase activity, which could be blocked by SCH 23390, a specific DA1 antagonist, and by PKI-(5-24) amide, a specific inhibitor of cAMP-dependent protein kinase. The results indicate that DA1 dopamine receptors and DARPP-32, an intracellular third messenger for dopamine, are part of the signal-transduction process for dopamine acting on renal tubule cells.

Original languageEnglish
Pages (from-to)8068-8072
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number20
DOIs
StatePublished - 1989
Externally publishedYes

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