Dog secretin: Sequence and biologic activity

Yasuhisa Shinomura, John Eng, Rosalyn S. Yalow

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Secretin was purified from the first 50 cm of proximal intestine of a single dog. The purification sequence included extraction in acid-ethanol, differential precipitation with acetone, Sephadex gel filtration and three successive HPLC steps. Dog secretin has the following sequence: HSDGTFTSELSRLESARLQRLLQGLV. The underlined amino acid represents a substitution of Glu for Asp at position 15 from the NH2-terminus of pig secretin. Dog secretin is equal in potency to pig secretin in stimulating pancreatic ductal flow in a rat bioassay system.

Original languageEnglish
Pages (from-to)1243-1248
Number of pages6
JournalLife Sciences
Issue number10
StatePublished - 7 Sep 1987
Externally publishedYes


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