DNA Vaccines in Pigs: From Immunization to Antigen Identification

Francesc Accensi, Laia Bosch-Camós, Paula L. Monteagudo, Fernando Rodríguez

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

DNA vaccination is one of the most fascinating vaccine strategies currently in development. Two of the main advantages of DNA immunization rely on its simplicity and flexibility, being ideal to dissect both the immune mechanisms and the antigens involved in protection against a given pathogen. Here we describe several strategies used to enhance the immune responses induced and the protection afforded by experimental DNA vaccines tested in swine and provide very basic protocols describing the generation and in vivo application of a prototypic DNA vaccine. The future will say the last word regarding the definitive implementation of DNA vaccination in the field.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages109-124
Number of pages16
DOIs
StatePublished - 2022
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2465
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • APC
  • Antibodies
  • Antigen presentation
  • Cytotoxic T-cells (CTL)
  • DNA vaccine
  • ELI
  • Electroporation
  • Genetic adjuvant
  • Swine
  • Veterinary virology

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