DNA repair after ultraviolet irradiation of ICR 2A frog cells. Pyrimidine dimers are long acting blocks to nascent DNA synthesis

B. S. Rosenstein; R. B. Setlow

doi:10.1016/S0006-3495(80)85050-8

DNA repair after ultraviolet irradiation of ICR 2A frog cells. Pyrimidine dimers are long acting blocks to nascent DNA synthesis

B. S. Rosenstein, R. B. Setlow

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33 Scopus citations

Abstract

The ability of ICR 2A frog cells to repair DNA damage induced by ultraviolet irradiation was examined. These cells are capable of photoreactivation but are nearly totally deficient in excision repair. They have the ability to convert the small molecule weight DNA made after irradiation into large molecules but do not show an enhancement in this process when the UV dose is delivered in two separate exposures separated by a 3- or 24-h incubation. Total DNA synthesis is depressed and low molecular weight DNA continues to be synthesized during pulse-labeling as long as 48 h after irradiation. The effects of pyrimidine dimer removal through exposure of UV irradiated cells to photoreactivating light indicate that dimers act as the critical lesions blocking DNA synthesis.

Original language	English
Pages (from-to)	195-205
Number of pages	11
Journal	Biophysical Journal
Volume	31
Issue number	2
DOIs	https://doi.org/10.1016/S0006-3495(80)85050-8
State	Published - 1980

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title = "DNA repair after ultraviolet irradiation of ICR 2A frog cells. Pyrimidine dimers are long acting blocks to nascent DNA synthesis",

abstract = "The ability of ICR 2A frog cells to repair DNA damage induced by ultraviolet irradiation was examined. These cells are capable of photoreactivation but are nearly totally deficient in excision repair. They have the ability to convert the small molecule weight DNA made after irradiation into large molecules but do not show an enhancement in this process when the UV dose is delivered in two separate exposures separated by a 3- or 24-h incubation. Total DNA synthesis is depressed and low molecular weight DNA continues to be synthesized during pulse-labeling as long as 48 h after irradiation. The effects of pyrimidine dimer removal through exposure of UV irradiated cells to photoreactivating light indicate that dimers act as the critical lesions blocking DNA synthesis.",

author = "Rosenstein, {B. S.} and Setlow, {R. B.}",

note = "Funding Information: This research was carried out at the Brookhaven National Laboratory under the auspices of the United States Department of Energy. B.S. Rosenstein is a Fellow in Cancer Research supported by grant DRG-215-F of the Damon Runyon-Walter Winchell Cancer Fund.",

year = "1980",

doi = "10.1016/S0006-3495(80)85050-8",

language = "English",

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T1 - DNA repair after ultraviolet irradiation of ICR 2A frog cells. Pyrimidine dimers are long acting blocks to nascent DNA synthesis

AU - Rosenstein, B. S.

AU - Setlow, R. B.

N1 - Funding Information: This research was carried out at the Brookhaven National Laboratory under the auspices of the United States Department of Energy. B.S. Rosenstein is a Fellow in Cancer Research supported by grant DRG-215-F of the Damon Runyon-Walter Winchell Cancer Fund.

PY - 1980

Y1 - 1980

N2 - The ability of ICR 2A frog cells to repair DNA damage induced by ultraviolet irradiation was examined. These cells are capable of photoreactivation but are nearly totally deficient in excision repair. They have the ability to convert the small molecule weight DNA made after irradiation into large molecules but do not show an enhancement in this process when the UV dose is delivered in two separate exposures separated by a 3- or 24-h incubation. Total DNA synthesis is depressed and low molecular weight DNA continues to be synthesized during pulse-labeling as long as 48 h after irradiation. The effects of pyrimidine dimer removal through exposure of UV irradiated cells to photoreactivating light indicate that dimers act as the critical lesions blocking DNA synthesis.

AB - The ability of ICR 2A frog cells to repair DNA damage induced by ultraviolet irradiation was examined. These cells are capable of photoreactivation but are nearly totally deficient in excision repair. They have the ability to convert the small molecule weight DNA made after irradiation into large molecules but do not show an enhancement in this process when the UV dose is delivered in two separate exposures separated by a 3- or 24-h incubation. Total DNA synthesis is depressed and low molecular weight DNA continues to be synthesized during pulse-labeling as long as 48 h after irradiation. The effects of pyrimidine dimer removal through exposure of UV irradiated cells to photoreactivating light indicate that dimers act as the critical lesions blocking DNA synthesis.

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DO - 10.1016/S0006-3495(80)85050-8

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JO - Biophysical Journal

JF - Biophysical Journal

IS - 2

ER -

DNA repair after ultraviolet irradiation of ICR 2A frog cells. Pyrimidine dimers are long acting blocks to nascent DNA synthesis

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