DNA preparation from schizosaccharomyces pombe

Assen Roguev; Jiewei Xu; Nevan J. Krogan

doi:10.1101/pdb.prot091959

DNA preparation from schizosaccharomyces pombe

Assen Roguev
, Jiewei Xu
, Nevan J. Krogan

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

This protocol includes two methods for genomic DNA preparation from Schizosaccharomyces pombe. The first is a quick method for preparation of DNA suitable for polymerase chain reaction (PCR) genotyping. The second, longer method yields high-quality DNA that can be used for amplification of targeting cassettes.

Original language	English
Pages (from-to)	44-46
Number of pages	3
Journal	Cold Spring Harbor Protocols
Volume	2018
Issue number	1
DOIs	https://doi.org/10.1101/pdb.prot091959
State	Published - Jan 2018
Externally published	Yes

Access to Document

10.1101/pdb.prot091959

Cite this

@article{7b4d7e3264fe4084886aabcdd482825e,

title = "DNA preparation from schizosaccharomyces pombe",

abstract = "This protocol includes two methods for genomic DNA preparation from Schizosaccharomyces pombe. The first is a quick method for preparation of DNA suitable for polymerase chain reaction (PCR) genotyping. The second, longer method yields high-quality DNA that can be used for amplification of targeting cassettes.",

author = "Assen Roguev and Jiewei Xu and Krogan, \{Nevan J.\}",

note = "Publisher Copyright: {\textcopyright} 2018 Cold Spring Harbor Laboratory Press.",

year = "2018",

month = jan,

doi = "10.1101/pdb.prot091959",

language = "English",

volume = "2018",

pages = "44--46",

journal = "Cold Spring Harbor Protocols",

issn = "1940-3402",

publisher = "Cold Spring Harbor Laboratory Press",

number = "1",

}

TY - JOUR

T1 - DNA preparation from schizosaccharomyces pombe

AU - Roguev, Assen

AU - Xu, Jiewei

AU - Krogan, Nevan J.

PY - 2018/1

Y1 - 2018/1

N2 - This protocol includes two methods for genomic DNA preparation from Schizosaccharomyces pombe. The first is a quick method for preparation of DNA suitable for polymerase chain reaction (PCR) genotyping. The second, longer method yields high-quality DNA that can be used for amplification of targeting cassettes.

AB - This protocol includes two methods for genomic DNA preparation from Schizosaccharomyces pombe. The first is a quick method for preparation of DNA suitable for polymerase chain reaction (PCR) genotyping. The second, longer method yields high-quality DNA that can be used for amplification of targeting cassettes.

UR - https://www.scopus.com/pages/publications/85039872185

U2 - 10.1101/pdb.prot091959

DO - 10.1101/pdb.prot091959

M3 - Article

C2 - 28733411

AN - SCOPUS:85039872185

SN - 1940-3402

VL - 2018

SP - 44

EP - 46

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

IS - 1

ER -

DNA preparation from schizosaccharomyces pombe

Abstract

Access to Document

Fingerprint

Cite this